Vitamin D Receptor antibody (C-Term)

Catalog No. ABIN3043960

Product Details anti-Vitamin D Receptor Antibody

Target: Vitamin D Receptor (VDR)

Binding Specificity: AA 377-402, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vitamin D Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Vitamin D3 receptor(VDR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: HLLYAKMIQK LADLRSLNEE HSKQYR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Vitamin D3 receptor(VDR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: vitamin D (1,25- dihydroxyvitamin D3) receptor
Protein Name: Vitamin D3 receptor

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VDR (377-402aa HLLYAKMIQKLADLRSLNEEHSKQYR), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for VDR is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Vitamin D Receptor antibody (ABIN3043960)

Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Gao, Wang, Yan, Zeng, Ma, Niu, Zhou, Jiang, Chen: "Comparative Transcriptome Analysis of Fetal Skin Reveals Key Genes Related to Hair Follicle Morphogenesis in Cashmere Goats." in: PLoS ONE, Vol. 11, Issue 3, pp. e0151118, (2016) (PubMed).

Target Details for Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Alternative Name: VDR (VDR Products)

Synonyms: vdrbeta antibody, vdr0 antibody, LOC100136219 antibody, NR1I1-B antibody, gb:dq017633 antibody, vdr-b antibody, Ci-VDR-b antibody, VDR antibody, LOC100221284 antibody, vdr-A antibody, xVDR antibody, Nr1i1 antibody, vdr antibody, NR1I1 antibody, PPP1R163 antibody, vitamin D (1,25- dihydroxyvitamin D3) receptor antibody, vitamin D receptor antibody, vitamin D3 receptor A antibody, vitamin D receptor b antibody, nuclear receptor VDR-b antibody, vitamin D (1,25- dihydroxyvitamin D3) receptor L homeolog antibody, vitamin D (1,25-dihydroxyvitamin D3) receptor antibody, vitamin D receptor a antibody, vdr antibody, vdr0 antibody, VDR antibody, LOC100136219 antibody, vdrb antibody, vdr-b antibody, vdr.L antibody, Vdr antibody, vdra antibody

Target Type: Chemical

Background: VDR (Vitamin D Receptor), also known as Vitamin D Hormone Receptor, is a member of the nuclear receptor family of transcription factors. Labuda et al. (1991) assigned the VDR gene to 12q12-q14 by in situ hybridization. Using mutation analysis, Jurutka et al. (2000) characterized arg18/arg22, VDR residues immediately N-terminal of the first DNA-binding zinc finger, as vital for contact with the general transcription factor IIB (TFIIB). A natural polymorphic variant of VDR, termed F/M4 (missing a FokI restriction site), which lacks only the first 3 amino acids (including glu2), interacted more efficiently with TFIIB and also possessed elevated transcriptional activity compared with the full-length (f/M1) receptor. Shah et al. (2006) stated that the signaling and oncogenic activity of beta-catenin (CTNNB1) can be repressed by activation of VDR. Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25- dihydroxyvitamin D3.

Synonyms: 1 25 dihydroxyvitamin D3 receptor antibody|1 antibody|1,25-@dihydroxyvitamin D3 receptor antibody|125 dihydroxyvitamin D3 receptor antibody|25-dihydroxyvitamin D3 receptor antibody|Member 1 antibody|NR1I1 antibody|Nuclear receptor subfamily 1 group I member 1 antibody|VDR antibody|VDR_HUMAN antibody|Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody|Vitamin D hormone receptor antibody|Vitamin D nuclear receptor variant 1 antibody| Vitamin D receptor antibody|Vitamin D3 receptor antibody

Gene ID: 7421

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway