Versican antibody (N-Term)

Catalog No. ABIN3043957

Product Details anti-Versican Antibody

Target: Versican (Vcan)

Binding Specificity: AA 65-92, N-Term

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Versican antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Versican core protein(VCAN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KWSKIEVDKN GKDLKETTVL VAQNGNIK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Versican core protein(VCAN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: versican
Protein Name: Versican core protein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Versican (65-92aa KWSKIEVDKNGKDLKETTVLVAQNGNIK), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human , The detection limit for Versican is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Versican antibody (ABIN3043957)

Fujii, Karpova, Asagoe, Georgiev, Dummer, Urosevic-Maiwald: "Versican upregulation in Sézary cells alters growth, motility and resistance to chemotherapy." in: Leukemia, Vol. 29, Issue 10, pp. 2024-32, (2015) (PubMed).

Target Details for Versican

Target: Versican (Vcan)

Alternative Name: VCAN (Vcan Products)

Synonyms: br146 antibody, cspg2 antibody, cspg2a antibody, sb:cb63 antibody, versican antibody, CSPG2 antibody, Cspg2 antibody, wgn antibody, ervr antibody, pg-m antibody, wgn1 antibody, Xversican antibody, dcan antibody, cspg2b antibody, fb99b07 antibody, wu:fb99b07 antibody, ERVR antibody, GHAP antibody, PG-M antibody, WGN antibody, WGN1 antibody, 5430420N07Rik antibody, 9430051N09 antibody, DPEAAE antibody, NG2 antibody, PG-M(V0) antibody, PG-M(V1) antibody, hdf antibody, versican a antibody, versican antibody, versican b antibody, vcana antibody, VCAN antibody, Vcan antibody, vcan antibody, vcanb antibody

Background: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000 kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.

Synonyms: Chondroitin sulfate proteoglycan 2 antibody|Chondroitin sulfate proteoglycan core protein 2 antibody|CSPG2 antibody|CSPG2_HUMAN antibody|ERVR antibody|GHAP antibody|Glial hyaluronate binding protein antibody|Glial hyaluronate-binding protein antibody|Large fibroblast proteoglycan antibody|PG-M antibody|PGM antibody|VCAN antibody|Versican core protein antibody|Versican proteoglycan antibody|WGN 1 antibody|WGN antibody|WGN1 antibody

Gene ID: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process