ULK3 antibody (C-Term)

Catalog No. ABIN3043954

Product Details anti-ULK3 Antibody

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Binding Specificity: AA 427-454, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase ULK3(ULK3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: HTEVQNLMAR AEYLKEQVKM RESRWEAD

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase ULK3(ULK3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: unc-51 like kinase 3
Protein Name: Serine/threonine-protein kinase ULK3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human ULK3 (427-454aa HTEVQNLMARAEYLKEQVKMRESRWEAD), different from the related mouse and rat sequences by four amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ULK3

Target: ULK3 (Unc-51-Like Kinase 3 (ULK3))

Alternative Name: ULK3 (ULK3 Products)

Synonyms: zgc:162196 antibody, 1200015E14Rik antibody, unc-51 like kinase 3 antibody, unc-51 like kinase 3 L homeolog antibody, unc-51-like kinase 3 antibody, ULK3 antibody, ulk3 antibody, Ulk3 antibody, ulk3.L antibody

Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.

Synonyms: DKFZP434C131 antibody|FLJ90566 antibody|Serine/threonine-protein kinase ULK3 antibody|Ulk3 antibody|ULK3_HUMAN antibody|unc 51 like kinase 3 (C. elegans) antibody|Unc-51-like kinase 3 antibody

Gene ID: 25989