Peroxiredoxin 5 antibody (AA 66-198)

Catalog No. ABIN3043906

Product Details anti-Peroxiredoxin 5 Antibody

Target: Peroxiredoxin 5 (PRDX5)

Binding Specificity: AA 66-198

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Peroxiredoxin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Peroxiredoxin-5, mitochondrial(PRDX5) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Peroxiredoxin-5, mitochondrial(PRDX5) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: peroxiredoxin 5
Protein Name: Peroxiredoxin-5, mitochondrial

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Peroxiredoxin 5 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Peroxiredoxin 5

Target: Peroxiredoxin 5 (PRDX5)

Alternative Name: PRDX5 (PRDX5 Products)

Synonyms: acr1 antibody, aoeb166 antibody, pmp20 antibody, prx-5 antibody, prx5 antibody, prxv antibody, sbbi10 antibody, GB16965 antibody, zgc:112318 antibody, PRDX5 antibody, CG 32920 antibody, CG 7217 antibody, CG32920 antibody, CG7217 antibody, Dmel\\CG7217 antibody, dPrx5 antibody, dprx5 antibody, ACR1 antibody, AOEB166 antibody, B166 antibody, PLP antibody, PMP20 antibody, PRDX6 antibody, PRXV antibody, prx-V antibody, AOPP antibody, Pmp20 antibody, Prdx6 antibody, PrxV antibody, Aoeb166 antibody, Prx V antibody, peroxiredoxin 5 L homeolog antibody, peroxiredoxin-5, mitochondrial antibody, peroxiredoxin 5 antibody, Peroxiredoxin 5 antibody, prdx5.L antibody, LOC552429 antibody, prdx5 antibody, PRDX5 antibody, Prx5 antibody, Prdx5 antibody

Background: PRDX5 (peroxiredoxin 5) also known as AOEB166, ACR1,B166, MGC117264, MGC142283, MGC142285, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. The PRDX5 gene is mapped to11q13.1. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. PRDX5 plays a protective role against oxidative stress in human cartilage.

Synonyms: ACR 1 antibody|ACR1 antibody|Alu co repressor 1 antibody|Alu co-repressor 1 antibody|Alu corepressor 1 antibody|antioxidant enzyme B166 antibody|AOEB166 antibody|B166 antibody|liver tissue 2D-page spot 71B antibody|MGC117264 antibody|MGC142283 antibody|MGC142285 antibody|mitochondrial antibody|Peroxiredoxin 5 mitochondrial antibody|Peroxiredoxin 5, mitochondrial antibody|Peroxiredoxin V antibody|Peroxiredoxin-5 antibody|Peroxiredoxin5 antibody|peroxisomal antioxidant enzyme antibody|PLP antibody|PMP 20 antibody|PMP20 antibody|PRDX 5 antibody|PRDX 6 antibody|PRDX5 antibody|PRDX5_HUMAN antibody|PRDX6 antibody|Prx V antibody|Prx-V antibody|PRXV antibody|SBBI10 antibody|thioredoxin peroxidase PMP20 antibody|Thioredoxin reductase antibody|TPx type VI antibody

Gene ID: 25824

UniProt: P30044

Pathways: Cell RedoxHomeostasis