Peroxiredoxin 1 antibody (Middle Region)

Catalog No. ABIN3043903

Product Details anti-Peroxiredoxin 1 Antibody

Target: Peroxiredoxin 1 (PRDX1)

Binding Specificity: AA 100-128, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Peroxiredoxin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Peroxiredoxin-1(PRDX1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: MNIPLVSDPK RTIAQDYGVL KADEGISFR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Peroxiredoxin-1(PRDX1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: peroxiredoxin 1
Protein Name: Peroxiredoxin-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Peroxiredoxin 1 (100-128aa MNIPLVSDPKRTIAQDYGVLKADEGISFR), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Peroxiredoxin 1 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Peroxiredoxin 1

Target: Peroxiredoxin 1 (PRDX1)

Alternative Name: PRDX1 (PRDX1 Products)

Synonyms: MSP23 antibody, NKEF-A antibody, NKEFA antibody, PAG antibody, PAGA antibody, PAGB antibody, PRX1 antibody, PRXI antibody, TDPX2 antibody, NkefA antibody, OSF-3 antibody, OSF3 antibody, Paga antibody, PrdxI antibody, PrxI antibody, TDX2 antibody, TPxA antibody, Tdpx2 antibody, prx1 antibody, Hbp23 antibody, MGC80194 antibody, MGC84820 antibody, PRDX1 antibody, msp23 antibody, nkefa antibody, pag antibody, paga antibody, pagb antibody, prx-1 antibody, prxi antibody, tdpx2 antibody, hm:zehl0637 antibody, zgc:110343 antibody, TPX-2 antibody, peroxiredoxin 1 antibody, peroxiredoxin 1 S homeolog antibody, peroxiredoxin-1 pseudogene antibody, PRDX1 antibody, Prdx1 antibody, prdx1.S antibody, prdx1 antibody, LOC100350232 antibody

Background: PRDX1(Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The PRDX1 gene is mapped on 1p34.1. Prdx1 was expressed in differentiating motor neuron cells in developing embryonic chicken and mouse spinal cords. mmunoprecipitation analysis showed that GDE2 interacted directly with PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells. This protein may have a proliferative effect and play a role in cancer development or progression. In differentiating spinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between the Gde2 N- and C-terminal domains. An intramolecular disulfide bond between the GDE2 N- and C-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 for the induction of motor neuron differentiation.

Synonyms: Hbp23 antibody|Heme binding 23 kDa protein antibody|Macrophage 23 Kd stress protein antibody|Macrophage 23 kDa stress protein antibody|Macrophase stress protein 22 kDa antibody|Macrophase stress protein 23 kd antibody|MSP23 antibody|Natural killer cell enhancing factor A antibody|Natural killer cell-enhancing factor A antibody|Natural killer enhancing factor A antibody|NKEF A antibody|NKEF-A antibody|NkefA antibody|OSF3 antibody|Osteoblast specific factor 3 antibody|PAG antibody|Paga antibody|PAGB antibody|Peroxiredoxin-1 antibody|Prdx 1 antibody|Prdx1 antibody|PRDX1_HUMAN antibody|Proliferation associated gene A antibody|Proliferation associated protein PAG antibody|Proliferation-associated gene protein antibody|PrxI antibody|Tdpx 2 antibody|Tdpx2 antibody|TDX2 antibody|Thioredoxin dependent peroxide reductase 2 antibody|Thioredoxin peroxidase 2 antibody|Thioredoxin-dependent peroxide reductase 2 antibody|TPxA antibody|Trx dependent peroxide reductase 2 antibody

Gene ID: 5052

UniProt: Q06830

Pathways: p53 Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events