POMC antibody (Middle Region)

Catalog No. ABIN3043901

Product Details anti-POMC Antibody

Target: POMC (Proopiomelanocortin (POMC))

Binding Specificity: AA 138-176, Middle Region

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POMC antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Pro-opiomelanocortin(POMC) detection. Tested with IHC-P in Human,Mouse,Rat.

Sequence: SYSMEHFRWG KPVGKKRRPV KVYPNGAEDE SAEAFPLEF

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Pro-opiomelanocortin(POMC) detection. Tested with IHC-P in Human,Mouse,Rat.
Gene Name: proopiomelanocortin
Protein Name: Pro-opiomelanocortin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ACTH(138-176aa SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-POMC antibody (ABIN3043901)

Huo, Liu, Wang, Zhang, Wang, Yang, Sun, Xu: "Early hyperbaric oxygen therapy inhibits aquaporin 4 and adrenocorticotropic hormone expression in the pituitary gland of rabbits with blast-induced craniocerebral injury." in: Neural regeneration research, Vol. 7, Issue 22, pp. 1729-35, (2015) (PubMed).

Chen, Wang, Zhao, Zhang, Huang: "Effects of the extract of a Chinese herb Tripterygium wilfordii hook f on rat pituitary gland." in: The American journal of Chinese medicine, Vol. 33, Issue 6, pp. 945-55, (2006) (PubMed).

Target Details for POMC

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: POMC (POMC Products)

Synonyms: MSH antibody, ACTH antibody, pomc antibody, SI:bZ36D5.1 antibody, SI:bZ36D5.4 antibody, si:bz36d5.2 antibody, BE antibody, Beta-LPH antibody, Clip antibody, Gamma-LPH antibody, Npp antibody, Pomc-1 antibody, Pomc1 antibody, alpha-MSH antibody, alphaMSH antibody, beta-MSH antibody, gamma-MSH antibody, CLIP antibody, LPH antibody, NPP antibody, POC antibody, Pomc2 antibody, proopiomelanocortin a antibody, pro-opiomelanocortin-alpha antibody, proopiomelanocortin antibody, pomca antibody, Pomc antibody, POMC antibody

Background: Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with its precursor corticotropin-releasing hormone from the hypothalamus). Its principal effects are increased production and release of corticosteroids. ACTH stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal glands. This gene can influence steroid hormone secretion by both rapid short-term mechanisms that take place within minutes and slower long-term actions. Besides, ACTH also enhances transcription of mitochondrial genes that encode for subunits of mitochondrial oxidative phosphorylation systems.

Synonyms: ACTH antibody|Adrenocorticotropic hormone antibody|Adrenocorticotropin antibody|Adrenocorticotropin Hormone antibody|Alpha Melanocyte Stimulating Hormone antibody|alpha-MSH antibody|alphaMSH antibody|Beta Endorphin antibody|Beta Lipotropin antibody|Beta LPH antibody|Beta Melanocyte Stimulating Hormone antibody|Beta-endorphin antibody|beta-MSH antibody|CLIP antibody|Corticotropin antibody|Corticotropin Like Intermediary Peptide antibody|Corticotropin lipotropin antibody|Corticotropin lipotropin precursor antibody|Corticotropin-like intermediary peptide antibody|Gamma LPH antibody|gamma-MSH antibody|Lipotropin Beta antibody|Lipotropin Gamma antibody|Lipotropin, included antibody|LPH antibody|Melanocyte-stimulating hormone, included antibody|Melanotropin Alpha antibody|Melanotropin beta antibody|Melanotropin gamma antibody|Melanotropin, included antibody|Met Enkephalin antibody|Met-enkephalin antibody|MSH antibody|NPP antibody|Opiomelanocortin prepropeptide antibody|POC antibody|POMC antibody|Pomc-1 antibody|Pomc1 antibody|Pomc2 antibody|Pro ACTH endorphin antibody|Pro opiomelanocortin antibody|Pro-opiomelanocortin-alpha antibody|Proopiomelanocortin antibody|Proopiomelanocortin preproprotein antibody|Tetracosactide antibody

Gene ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour