PGRMC1 antibody (Middle Region)
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- Target See all PGRMC1 Antibodies
- PGRMC1 (Progesterone Receptor Membrane Component 1 (PGRMC1))
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Binding Specificity
- AA 67-102, Middle Region
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PGRMC1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Membrane-associated progesterone receptor component 1(PGRMC1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- RLKRRDFTPA ELRRFDGVQD PRILMAINGK VFDVTK
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Membrane-associated progesterone receptor component 1(PGRMC1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: progesterone receptor membrane component 1
Protein Name: Membrane-associated progesterone receptor component 1 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human PGRMC1 (67-102aa RLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTK), identical to the related mouse sequence, and different from the related rat sequence by two amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product PGRMC1 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- PGRMC1 (Progesterone Receptor Membrane Component 1 (PGRMC1))
- Alternative Name
- PGRMC1 (PGRMC1 Products)
- Synonyms
- MGC89150 antibody, wu:fa94d03 antibody, zgc:103577 antibody, DKFZp469D0815 antibody, HPR6.6 antibody, MPR antibody, AA415812 antibody, PPMR antibody, Vema antibody, 25-Dx antibody, 25Dx antibody, VEMA antibody, progesterone receptor membrane component 1 antibody, pgrmc1 antibody, PGRMC1 antibody, Pgrmc1 antibody
- Background
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Progesterone receptor membrane component 1 (PGRMC1) is a protein which co-purifies with progesterone binding proteins in the liver and ovary. In humans, the PGRMC1 protein is encoded by the PGRMC1 gene. The Sigma-2 receptor was recently identified as potentially being the same as PGRMC1. The sole biochemical function of PGRMC1 is heme-binding. PGRMC1 shares key structural motifs with cytochrome b5. It binds and activates P450 proteins, which are important in drug, hormone and lipid metabolism. Also, PGRMC1 binds to PAIR-BP1 (plasminogen activator inhibitor RNA-binding protein-1). However, its expression outside of the reproductive tract and in males suggests multiple functions for the protein. These may include binding to Insig (insulin-induced gene), which regulates cholesterol synthesis.
Synonyms: HPR6.6 antibody|Membrane associated progesterone receptor component 1 antibody|Membrane-associated progesterone receptor component 1 antibody|mPR antibody|PGRC1_HUMAN antibody|PGRMC antibody|Pgrmc1 antibody|Progesterone binding protein antibody|Progesterone receptor membrane component 1 antibody - Gene ID
- 10857
- UniProt
- O00264
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