MYD88 antibody (AA 44-264)

Catalog No. ABIN3043887

Product Details anti-MYD88 Antibody

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Binding Specificity: AA 44-264

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYD88 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Myeloid differentiation primary response protein MyD88(MYD88) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Myeloid differentiation primary response protein MyD88(MYD88) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: myeloid differentiation primary response 88
Protein Name: Myeloid differentiation primary response protein MyD88

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MyD88 recombinant protein (Position: A44-F264). Human MyD88 shares 84% and 83% amino acid (aa) sequences identity with mouse and rat MyD88, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for MyD88 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-MYD88 antibody (ABIN3043887)

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Wang, Yuan, Wang, Yang, Chen, Liu, Song, Feng, Tan, Jia: "Anti-inflammatory Effects of Phyllanthus emblica L on Benzopyrene-Induced Precancerous Lung Lesion by Regulating the IL-1β/miR-101/Lin28B Signaling Pathway." in: Integrative cancer therapies, Vol. 16, Issue 4, pp. 505-515, (2018) (PubMed).

Song, Hu, Li, Zheng, Wang, Guo, Shi, Liu: "Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication." in: Archives of virology, Vol. 163, Issue 1, pp. 135-144, (2018) (PubMed).

Wang, Duan, Wu, Min, Huang, Luo, He: "Effect of cyclooxygenase‑2 inhibition on the development of post‑traumatic stress disorder in rats." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 4925-4932, (2018) (PubMed).

Sun, Xue, Xue, Ren, Wu, Wang: "Acetylpuerarin protects against OGD-induced cell injury in BV2 microglia by inhibiting HMGB1 release." in: Die Pharmazie, Vol. 73, Issue 2, pp. 92-97, (2018) (PubMed).

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Ahmadieh, Nourinia, Hafezi-Moghadam, Sabbaghi, Nakao, Zandi, Yaseri, Tofighi, Akbarian: "Intravitreal injection of a Rho-kinase inhibitor (fasudil) combined with bevacizumab versus bevacizumab monotherapy for diabetic macular oedema: a pilot randomised clinical trial." in: The British journal of ophthalmology, (2018) (PubMed).

Alsaegh, Miyashita, Taniguchi, Zhu: "Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma." in: Experimental and therapeutic medicine, Vol. 13, Issue 1, pp. 247-253, (2017) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, (2017) (PubMed).

Jiang, Tian, Tang, Ou, Xu et al.: "Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner ..." in: Mediators of inflammation, Vol. 2015, pp. 601604, (2016) (PubMed).

Lin, Huang, Shen, Yiming: "MicroRNA-101 regulates the viability and invasion of cervical cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10148-55, (2016) (PubMed).

Shen, Chen, Zhang, Du, Bai, Zhang, Jiang, Li, Wang, Zhu: "MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148532, (2016) (PubMed).

Zhao, Zhao, Wang, Li, Zhang: "Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 8219287, (2016) (PubMed).

Zhen, Zong, Gao, Cao, Jiang, Chen, Wang, Sun, Peng, Bai, Li: "Preparation and characterization of a novel aspirin derivative with anti-thrombotic and gastric mucosal protection properties." in: PLoS ONE, Vol. 9, Issue 6, pp. e98513, (2015) (PubMed).

Zhou, Wang, Liu, Hu, Zhu, Shen, Yao: "Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response." in: Neural regeneration research, Vol. 9, Issue 23, pp. 2074-80, (2015) (PubMed).

Wang, Yang, Cui, Chen, Jiang: "Anti-inflammatory and anti-nociceptive activities of methanol extract from aerial part of Phlomis younghusbandii Mukerjee." in: PLoS ONE, Vol. 9, Issue 3, pp. e89149, (2014) (PubMed).

Cao, Zhang, Xie, Jiang, Ji, Gao: "Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2." in: Experimental neurology, Vol. 261, pp. 328-36, (2014) (PubMed).

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Target Details for MYD88

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Alternative Name: MYD88 (MYD88 Products)

Synonyms: MYD88D antibody, XMyD88 antibody, myd88d antibody, MGC84928 antibody, CG2078 antibody, DMMYD88 antibody, DmMyD88 antibody, DmMyd88 antibody, Dmel\\CG2078 antibody, EP(2)2535 antibody, Kra antibody, LD20892 antibody, MYD88 antibody, MyD88 antibody, Myd88F antibody, dMyD88 antibody, dMyd88 antibody, kra antibody, myd88 antibody, zgc:103541 antibody, GB12344 antibody, NV10640 antibody, myd88-a antibody, myd88-b antibody, myeloid differentiation primary response 88 antibody, myeloid differentiation primary response 88 S homeolog antibody, myeloid differentiation primary response gene 88 antibody, CG2078 gene product from transcript CG2078-RB antibody, myeloid differentiation primary response protein MyD88-A antibody, myeloid differentiation primary response protein MyD88 antibody, myeloid differentiation factor 88 antibody, myeloid differentiation primary response 88 L homeolog antibody, MYD88 antibody, myd88.S antibody, Myd88 antibody, myd88 antibody, LOC413194 antibody, LOC100118553 antibody, LOC575153 antibody, myd88.L antibody

Background: MYD88(MYELOID DIFFERENTIATION PRIMARY RESPONSE GENE 88), is a protein that, in humans, is encoded by the MYD88 gene. MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL1Rs). And it is mapped on 3p22.2. MYD88 encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. Overexpression of MYD88 caused an increase in the level of transcription from the interleukin-8 promoter. The C-terminal domain of MYD88 has significant sequence similarity to the cytoplasmic domain of IL1RAP. Inhibiting the IL1R-MYD88 pathway in vivo could block the damage from acute inflammation that occurs in response to sterile cell death, and do so in a way that might not compromise tissue repair or host defense against pathogens.

Synonyms: MYD 88 antibody|Myd88 antibody|MYD88_HUMAN antibody|MYD88D antibody|Myeloid differentiation marker 88 antibody|Myeloid differentiation primary response gene 88 antibody|Myeloid differentiation primary response gene antibody|Myeloid differentiation primary response protein MyD88 antibody|OTTHUMP00000161718 antibody|OTTHUMP00000208595 antibody|OTTHUMP00000209058 antibody|OTTHUMP00000209059 antibody|OTTHUMP00000209060 antibody

Gene ID: 4615

UniProt: Q99836

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades