MCL-1 antibody (AA 1-350)

Catalog No. ABIN3043876

Product Details anti-MCL-1 Antibody

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Binding Specificity: AA 1-350

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Induced myeloid leukemia cell differentiation protein Mcl-1(MCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Induced myeloid leukemia cell differentiation protein Mcl-1(MCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: myeloid cell leukemia 1
Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for MCL1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-MCL-1 antibody (ABIN3043876)

Liu, Chen, Wang, Yang, Xue, Zhu: "Msi1 confers resistance to TRAIL by activating ERK in liver cancer cells." in: FEBS letters, Vol. 589, Issue 8, pp. 897-903, (2015) (PubMed).

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL1 (MCL1 Products)

Synonyms: BCL2L3 antibody, EAT antibody, MCL1-ES antibody, MCL1L antibody, MCL1S antibody, Mcl-1 antibody, TM antibody, bcl2-L-3 antibody, mcl1/EAT antibody, AW556805 antibody, mcl-1 antibody, fb72f02 antibody, wu:fb70a08 antibody, wu:fb72f02 antibody, zfMLP1 antibody, zfMcl-1a antibody, zgc:109835 antibody, zgc:109763 antibody, E3 antibody, E3-2 antibody, RNE3 antibody, RNU107 antibody, SNORA63A antibody, elF-4AII antibody, MCL1, BCL2 family apoptosis regulator antibody, myeloid cell leukemia sequence 1 antibody, MCL1, BCL2 family apoptosis regulator a antibody, MCL1, BCL2 family apoptosis regulator b antibody, small nucleolar RNA, H/ACA box 63 antibody, MCL1 antibody, Mcl1 antibody, mcl1a antibody, mcl1b antibody, SNORA63 antibody

Background: MCL1, also known as myeloid cell leukemia 1, is a protein that in humans is encoded the MCL1 gene. It is mapped to 1q21.3. MCL1 is a potent multidomain antiapoptotic protein of the BCL2 family that heterodimerizes with other BCL2 family members to protect against apoptotic cell death. MCL1 as an attractive candidate for regulation of hematopoietic stem cell homeostasis that is highly expressed in hematopoietic stem cells and regulated by growth factor signals. MCL1 is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. During mitotic arrest, MCL1 protein levels decline markedly, through a posttranslational mechanism, potentiating cell death. Phosphorylation of MCL1 directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex.

Synonyms: Bcl 2 related protein EAT/mcl1 antibody|Bcl-2-like protein 3 antibody|Bcl-2-related protein EAT/mcl1 antibody|BCL2 related antibody|Bcl2-L-3 antibody|BCL2L3 antibody|EAT antibody|Induced myeloid leukemia cell differentiation protein Mcl 1 antibody|Induced myeloid leukemia cell differentiation protein Mcl-1 antibody|MCL 1 antibody|MCL1 antibody|MCL1-ES antibody|Mcl1/EAT antibody|MCL1_HUMAN antibody|MCL1L antibody|MCL1S antibody|MGC104264 antibody|MGC1839 antibody|Myeloid Cell Leukemia 1 antibody|Myeloid cell leukemia ES antibody|myeloid cell leukemia sequence 1 antibody|Myeloid cell leukemia sequence 1 BCL2 related antibody|Myeloid cell leukemia sequence 1 isoform 1 antibody|OTTHUMP00000032794 antibody|OTTHUMP00000032795 antibody|TM antibody

Gene ID: 4170

UniProt: Q07820

Pathways: MAPK Signaling