KCNMA1 antibody (AA 124-467)

Catalog No. ABIN3043867

This Rabbit Polyclonal antibody specifically detects KCNMA1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for KCNMA1 antibody (AA 124-467) (ABIN3043867)

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KCNMA1 Antibody

Binding Specificity: AA 124-467

Purpose: Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband® (ABIN3043867). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KCNMA1 recombinant protein (Position: K124-Q467). Human KCNMA1 shares 99% amino acid (aa) sequence identity with both mouse and rat KCNMA1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: KCNMA1 potassium large conductance calcium-activated channel, subfamily M, alpha member 1" 2. Miller C (2000). "An overview of the potassium channel family". Genome Biol. 1 (4): reviews0004.1-reviews0004.5. 3. Yuan P, Leonetti MD, Pico AR, Hsiung Y, MacKinnon R (July 2010). "Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution". Science 329 (5988): 182-6. Bibcode:2010Sci...329..182Y.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for KCNMA1

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Alternative Name: KCNMA1

Background:

Synonyms: Calcium-activated potassium channel subunit alpha-1,BK channel,BKCA alpha,Calcium-activated potassium channel, subfamily M subunit alpha-1,K (VCA)alpha,KCa1.1,Maxi K channel,MaxiK,Slo-alpha,Slo1,Slowpoke homolog,Slo homolog,hSlo,KCNMA1,KCNMA, SLO,

Tissue Specificity: Widely expressed. Except in myocytes, it is almost ubiquitously expressed. .

Background: Calcium-activated potassium channel subunit alpha-1 also known as KCa1.1, or BK channel for short, is a voltage gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. This gene is located on 10q22.3. BK channels are activated (opened) by changes in membrane electrical potential and/or by increases in concentration of intracellular calcium ion (Ca2+). It is essential for the regulation of several key physiological processes including smooth muscle tone and neuronal excitability. BK channels also contribute to the behavioral effects of ethanol in the worm C. elegans under high concentrations (> 100 mM, or approximately 0.50 % BAC).It remains to be determined if BK channels contribute to intoxication in humans.

Sequence Similarities: Belongs to the potassium channel family. Calcium- activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.

Molecular Weight: 110-138 kDa

Gene ID: 3778

UniProt: Q12791

Pathways: Regulation of Hormone Metabolic Process, Sensory Perception of Sound