HSP90AA1 antibody (AA 2-365)
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- Target See all HSP90AA1 Antibodies
- HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
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Binding Specificity
- AA 2-365
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HSP90AA1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Purpose
- Rabbit IgG polyclonal antibody for Heat shock protein HSP 90-alpha(HSP90AA1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Heat shock protein HSP 90-alpha(HSP90AA1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: heat shock protein 90 kDa alpha (cytosolic), class A member 1
Protein Name: Heat shock protein HSP 90-alpha - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Hsp90 recombinant protein (Position: P2-V365). Human Hsp90 shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90.
- Isotype
- IgG
- Top Product
- Discover our top product HSP90AA1 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for Hsp90 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).
: "Heat shock protein 90-mediated inactivation of nuclear factor-?B switches autophagy to apoptosis through becn1 transcriptional inhibition in selenite-induced NB4 cells." in: Molecular biology of the cell, Vol. 22, Issue 8, pp. 1167-80, (2011) (PubMed).
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The effect of 5'-adenylic acid on hepatic proteome of mice radiated by 60Co γ-ray." in: International journal of molecular sciences, Vol. 15, Issue 1, pp. 186-202, (2014) (PubMed).
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- Target
- HSP90AA1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
- Alternative Name
- HSP90AA1 (HSP90AA1 Products)
- Synonyms
- EL52 antibody, HSP86 antibody, HSP89A antibody, HSP90A antibody, HSP90N antibody, HSPC1 antibody, HSPCA antibody, HSPCAL1 antibody, HSPCAL4 antibody, HSPN antibody, Hsp89 antibody, Hsp90 antibody, LAP2 antibody, Hsp86 antibody, Hspca antibody, htpG antibody, 86kDa antibody, 89kDa antibody, AL024080 antibody, AL024147 antibody, Hsp86-1 antibody, hsp4 antibody, HSP90 antibody, HSP90AA1 antibody, fb17b01 antibody, hsp90 antibody, hsp90a antibody, hsp90a.1 antibody, hsp90alpha antibody, wu:fb17b01 antibody, zgc:86652 antibody, Hsp90alpha antibody, heat shock protein 90 alpha family class A member 1 antibody, heat shock protein 90, alpha (cytosolic), class A member 1 antibody, Heat Shock Protein 90, cytosolic antibody, heat shock protein 90A antibody, molecular chaperone antibody, heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1 antibody, heat shock protein HSP 90-alpha antibody, heat shock protein 90kDa alpha (cytosolic), class A member 1 antibody, HSP90AA1 antibody, Hsp90aa1 antibody, HSP90A antibody, hsp90A antibody, hsp90aa1.1 antibody, LOC108698781 antibody
- Background
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Heat shock protein HSP 90-alpha is a protein that in humans is encoded by the HSP90AA1 gene. The gene, HSP90AA1, encodes the human stress-inducible 90- kDa heat shock protein alpha (Hsp90A). Complemented by the constitutively expressed paralog Hsp90B which shares over 85 % amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress. Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures. This molecular chaperoning ability of Hsp90A is driven by a cycle of structural rearrangements fueled by ATP hydrolysis. Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation.
Synonyms: D6S182 antibody|FLJ26984 antibody|FLJ31884 antibody|Heat shock 86 kDa antibody|heat shock 90 kDa protein 1 alpha antibody|Heat shock protein 90 kDa alpha cytosolic class A member 1 antibody|Heat shock protein 90 kDa alpha cytosolic class B member 1 antibody|Heat shock protein HSP 90 alpha antibody|Heat shock protein HSP 90 beta antibody|Heat shock protein HSP 90-alpha antibody|HS90A_HUMAN antibody|HSP 84 antibody|HSP 86 antibody|Hsp 90 antibody|HSP84 antibody|HSP86 antibody|Hsp89 antibody|Hsp90 antibody|HSP90 Beta antibody|HSP90A antibody|HSP90AA1 antibody|HSP90AB1 antibody|HSP90B antibody|HSP90N antibody|HSPC1 antibody|HSPC2 antibody|HSPCA antibody|HSPCAL1 antibody|HSPCAL4 antibody|HSPCB antibody|HSPN antibody|LAP2 antibody|Lipopolysaccharide associated protein2 antibody|LPS associated protein 2 antibody|NY REN 38 antigen antibody|Renal carcinoma antigen NY-REN-38 antibody - Gene ID
- 3320
- UniProt
- P07900
- Pathways
- M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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