CD3 epsilon antibody (AA 23-207)

Catalog No. ABIN3043805

Product Details anti-CD3 epsilon Antibody

Target: CD3 epsilon (CD3E)

Binding Specificity: AA 23-207

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD3 epsilon chain(CD3E) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: CD3e molecule, epsilon (CD3-TCR complex)
Protein Name: T-cell surface glycoprotein CD3 epsilon chain

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD3 epsilon is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD3 epsilon antibody (ABIN3043805)

Guo, Qian, Zhang: "The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis." in: BMC ophthalmology, Vol. 16, pp. 66, (2017) (PubMed).

Chen, Liu, Xu, Yang, Zhang, Zhang: "Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 1063-1067, (2015) (PubMed).

Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, (2013) (PubMed).

Li, Geng, Yin, Chen, Yang, Wu, Li, Du, Schmidt, Przybylski: "Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients." in: Journal of translational medicine, Vol. 8, pp. 47, (2010) (PubMed).

Xie, Jia, He, Gao: "Morphological observation of tumor infiltrating immunocytes in human rectal cancer." in: World journal of gastroenterology, Vol. 12, Issue 11, pp. 1757-60, (2006) (PubMed).

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E (CD3E Products)

Background: CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Synonyms: CD3 epsilon antibody|CD3e antibody|CD3E antibody|CD3e antigen epsilon polypeptide (TiT3 complex) antibody|CD3E antigen epsilon polypeptide antibody|CD3E antigen, epsilon subunit antibody|CD3e molecule epsilon antibody|CD3e molecule, epsilon (CD3 TCR complex) antibody|CD3e molecule, epsilon (CD3-TCR complex) antibody|CD3E_HUMAN antibody|IMD18 antibody|T cell antigen receptor complex epsilon subunit of T3 antibody|T cell surface antigen T3/Leu 4 epsilon chain antibody|T cell surface glycoprotein CD3 epsilon chain antibody|T-cell surface antigen T3/Leu-4 epsilon chain antibody|T-cell surface glycoprotein CD3 epsilon chain antibody|T3E antibody|TCRE antibody

Gene ID: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway