ATF2 antibody (AA 93-450)

Catalog No. ABIN3043791

Product Details anti-ATF2 Antibody

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Binding Specificity: AA 93-450

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Cyclic AMP-dependent transcription factor ATF-2(ATF2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cyclic AMP-dependent transcription factor ATF-2(ATF2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: activating transcription factor 2
Protein Name: Cyclic AMP-dependent transcription factor ATF-2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATF2 recombinant protein (Position: E93-E450). Human ATF2 shares 99% amino acid (aa) sequence identity with both mouse and rat ATF2.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for ATF2 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Alternative Name: ATF2 (ATF2 Products)

Synonyms: CRE-BP1 antibody, CREB2 antibody, HB16 antibody, TREB7 antibody, CG12850 antibody, CG3749 antibody, dATF-2 antibody, DmelCG30420 antibody, CG30420 antibody, CHN1 antibody, Atf-2 antibody, CRE-BP antibody, Creb2 antibody, D130078H02Rik antibody, D18875 antibody, Tg(Gzma-Klra1)7Wum antibody, mXBP antibody, atf2-A antibody, atf2l antibody, activating transcription factor 2 antibody, Activating transcription factor-2 antibody, activating transcription factor 2 S homeolog antibody, ATF2 antibody, Atf-2 antibody, Atf2 antibody, atf2.S antibody, atf2 antibody

Background: ATF2, also known as Activating transcription factor 2, is a protein that in humans is encoded by the ATF2 gene. It is mapped to 2q31.1. This gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus, it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.

Synonyms: Activating Transcription Factor 2 antibody|Activating transcription factor 2 splice variant ATF2 var2 antibody|ATF 2 antibody|Atf-2 antibody|Atf2 antibody|ATF2 protein antibody|ATF2_HUMAN antibody|cAMP Response Element Binding Protein 2 antibody|cAMP response element binding protein CRE BP1 antibody|cAMP response element-binding protein CRE-BP1 antibody|cAMP responsive element binding protein 2, formerly antibody|cAMP-dependent transcription factor ATF-2 antibody|cAMP-responsive element-binding protein 2 antibody|CRE BP1 antibody|CRE-BP antibody|CREB 2 antibody|CREB-2 antibody|CREB2 antibody|CREBP1 antibody|Cyclic AMP dependent transcription factor ATF 2 antibody|Cyclic AMP-dependent transcription factor ATF-2 antibody|Cyclic AMP-responsive element-binding protein 2 antibody|D130078H02Rik antibody|D18875 antibody|HB 16 antibody|HB16 antibody|Histone acetyltransferase ATF2 antibody|MGC105211 antibody|MGC105222 antibody|MGC111558 antibody|MGC142504 antibody|mXBP antibody|MXBP protein antibody|Tg(Gzma-Klra1)7Wum antibody|TREB 7 antibody|TREB7 antibody

Gene ID: 1386

UniProt: P15336

Pathways: MAPK Signaling, RTK Signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades