Annexin a1 antibody (AA 2-346)

Catalog No. ABIN3043783

Product Details anti-Annexin a1 Antibody

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Binding Specificity: AA 2-346

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin a1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Annexin A1(ANXA1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Annexin A1(ANXA1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: annexin A1
Protein Name: Annexin A1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequences identity with mouse and rat Annexin A1, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Annexin A1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Annexin a1 antibody (ABIN3043783)

Zhang, Wu, Zha, Zhao, Feng: "The prognostic value and clinicopathological features of sarcomatoid differentiation in patients with renal cell carcinoma: a systematic review and meta-analysis." in: Cancer management and research, Vol. 10, pp. 1687-1703, (2018) (PubMed).

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." in: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Mohamed, Idris, Ahmed, Åstrøm, Mustafa, Ibrahim, Mustafa: "Influence of type 2 diabetes on local production of inflammatory molecules in adults with and without chronic periodontitis: a cross-sectional study." in: BMC oral health, Vol. 15, pp. 86, (2015) (PubMed).

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: ANXA1 (ANXA1 Products)

Synonyms: anx1a antibody, anxa1 antibody, wu:fa01d11 antibody, wu:fa05d11 antibody, wu:fk69h01 antibody, MGC64363 antibody, anx1 antibody, lpc1 antibody, MGC89164 antibody, ANXA1 antibody, ANX1 antibody, LPC1 antibody, p35 antibody, Anx-1 antibody, Anx-A1 antibody, C430014K04Rik antibody, Lpc-1 antibody, Lpc1 antibody, Anx1 antibody, annexin A1a antibody, annexin A1 antibody, annexin A1 L homeolog antibody, Annexin A1 antibody, anxa1a antibody, anxa1 antibody, anxa1.L antibody, ANXA1 antibody, LOC100335033 antibody, Anxa1 antibody

Background: ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Synonyms: Annexin 1 antibody|Annexin A1 antibody|Annexin I (lipocortin I) antibody|Annexin I antibody|Annexin-1 antibody|AnnexinA1 antibody|AnnexinI antibody|ANX 1 antibody|ANX A1 antibody|ANX1 antibody|ANXA 1 antibody|ANXA1 antibody|ANXA1 protein antibody|ANXA1_HUMAN antibody|Calpactin 2 antibody|Calpactin II antibody|Calpactin-2 antibody|CalpactinII antibody|Chromobindin 9 antibody|Chromobindin-9 antibody|Chromobindin9 antibody|Lipocortin 1 antibody|Lipocortin I antibody|Lipocortin1 antibody|LipocortinI antibody|LPC 1 antibody|LPC1 antibody|p35 antibody|Phospholipase A2 inhibitory protein antibody

Gene ID: 301

UniProt: P04083

Pathways: Hormone Transport