CALB1 antibody (AA 2-175)

Catalog No. ABIN3043771

Product Details anti-CALB1 Antibody

Target: CALB1 (Calbindin (CALB1))

Binding Specificity: AA 2-175

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Calbindin(CALB1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Calbindin(CALB1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: calbindin 1, 28 kDa
Protein Name: Calbindin

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with mouse and rat Calbindin.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for Calbindin is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CALB1

Target: CALB1 (Calbindin (CALB1))

Alternative Name: CALB1 (CALB1 Products)

Synonyms: CALB antibody, CG6702 antibody, Calb antibody, Cbp33 antibody, DCBP antibody, Dcbp antibody, Dcp antibody, Dmel\\CG6702 antibody, cbn antibody, cbp53E antibody, Calbindin antibody, CB antibody, Calb-1 antibody, Brain-2 antibody, CaBP28K antibody, calb1 antibody, CALB1 antibody, fj53a05 antibody, wu:fj53a05 antibody, calbindin 1 antibody, Calbindin 53E antibody, CALB1 antibody, Cbp53E antibody, Calb1 antibody, calb1 antibody

Background: Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98 % sequence homology and 91 % homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.

Synonyms: avian-type antibody|CAB27 antibody|CALB 1 antibody|CALB antibody|CALB1 antibody|CALB1_HUMAN antibody|Calbindin 1 28 kDa antibody|Calbindin antibody|Calbindin D28 antibody|D 28K antibody|D-28K antibody|D28K antibody|OTTHUMP00000166027 antibody|OTTHUMP00000225441 antibody|RTVL H protein antibody|Vitamin D dependent calcium binding protein antibody|Vitamin D dependent calcium binding protein avian type antibody|Vitamin D dependent calcium binding protein avian-type antibody|Vitamin D-dependent calcium-binding protein antibody

Gene ID: 793

UniProt: P05937