BUB1 antibody (AA 731-1085)

Catalog No. ABIN3043764

Product Details anti-BUB1 Antibody

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Binding Specificity: AA 731-1085

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Mitotic checkpoint serine/threonine-protein kinase BUB1(BUB1) detection. Tested with WB in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Mitotic checkpoint serine/threonine-protein kinase BUB1(BUB1) detection. Tested with WB in Human,Rat.
Gene Name: BUB1 mitotic checkpoint serine/threonine kinase
Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for Bub1 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: BUB1 (BUB1 Products)

Synonyms: bub1a antibody, bub1l antibody, hbub1 antibody, xbub1 antibody, BUB1A antibody, BUB1L antibody, hBUB1 antibody, AL022991 antibody, Bub1a antibody, C80208 antibody, D2Xrf87 antibody, BUB1 mitotic checkpoint serine/threonine kinase antibody, BUB1, mitotic checkpoint serine/threonine kinase antibody, BUB1 antibody, bub1 antibody, Bub1 antibody

Background: BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

Synonyms: Bub1 antibody|BUB1 budding uninhibited by benzimidazoles 1 homolog antibody|BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody|BUB1 mitotic checkpoint serine/threonine kinase antibody|BUB1, S. cerevisiae, homolog of antibody|BUB1_HUMAN antibody|BUB1A antibody|BUB1L antibody|Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody|Budding uninhibited by benzimidazoles 1 homolog antibody|Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody|hBUB1 antibody|Homolog of mitotic checkpoint gene BUB1 antibody|Mitotic checkpoint gene BUB1 antibody|Mitotic checkpoint serine/threonine protein kinase BUB1 antibody|Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody|Mitotic spindle checkpoint kinase antibody|Putative serine/threonine protein kinase antibody

Gene ID: 699

UniProt: O43683