ALOX5 antibody (AA 120-483)

Catalog No. ABIN3043727

Product Details anti-ALOX5 Antibody

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Binding Specificity: AA 120-483

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Arachidonate 5-lipoxygenase(ALOX5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Arachidonate 5-lipoxygenase(ALOX5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: arachidonate 5-lipoxygenase
Protein Name: Arachidonate 5-lipoxygenase

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALOX5 recombinant protein (Position: A120-R483). Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for ALOX5 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5 (ALOX5 Products)

Synonyms: 5-LO antibody, 5-LOX antibody, 5LPG antibody, LOG5 antibody, 5LO antibody, 5LX antibody, AI850497 antibody, F730011J02 antibody, LOX5A antibody, si:dkey-194n13.2 antibody, lox5 antibody, arachidonate 5-lipoxygenase antibody, arachidonate 5-lipoxygenase a antibody, Arachidonate 5-lipoxygenase antibody, ALOX5 antibody, Alox5 antibody, alox5a antibody, AFUA_4G02770 antibody, AOR_1_1362014 antibody, lox5 antibody

Background: Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.

Synonyms: 5 LO antibody|5 LOX antibody|5-lipoxygenase antibody|5-LO antibody|5-LOX antibody|5LOX antibody|5LPG antibody|ALOX 5 antibody|Alox5 antibody|Arachidonate 5 lipoxygenase antibody|Arachidonate 5-lipoxygenase antibody|arachidonic 5-lipoxygenase alpha-10 isoform antibody|arachidonic 5-lipoxygenase delta-10-13 isoform antibody|arachidonic 5-lipoxygenase delta-13 isoform antibody|arachidonic 5-lipoxygenase delta-p10 isoform antibody|Arachidonic acid 5 lipoxygenase antibody|Leukotriene A4 synthase antibody|LOG 5 antibody|LOG5 antibody|LOX5_HUMAN antibody|MGC163204 antibody

Gene ID: 240

UniProt: P09917