Arylsulfatase A antibody (C-Term)

Catalog No. ABIN3043722

Product Details anti-Arylsulfatase A Antibody

Target: Arylsulfatase A (ARSA)

Binding Specificity: AA 454-482, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Arylsulfatase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Arylsulfatase A(ARSA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: QALKQLQLLK AQLDAAVTFG PSQVARGED

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Arylsulfatase A(ARSA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: arylsulfatase A
Protein Name: Arylsulfatase A

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA (454-482aa QALKQLQLLKAQLDAAVTFGPSQVARGED), different from the related mouse sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Arylsulfatase A

Target: Arylsulfatase A (ARSA)

Alternative Name: ARSA (ARSA Products)

Synonyms: ARSA antibody, zgc:101575 antibody, arsa antibody, AS-A antibody, ASA antibody, AW212749 antibody, As-2 antibody, As2 antibody, TISP73 antibody, MLD antibody, mld antibody, arylsulfatase A antibody, arylsulfatase antibody, arylsulfatase A, gene 1 S homeolog antibody, ARSA antibody, arsa antibody, arsA antibody, RB6599 antibody, Arsa antibody, arsa.1.S antibody

Background: Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. ARSA is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.

Synonyms: arsA antibody|ARSA_HUMAN antibody|arylsulfatase A antibody|Arylsulfatase A component C antibody|As 2 antibody|AS A antibody|As2 antibody|ASA antibody|AW212749 antibody| C230037L18Rik antibody|Cerebroside-sulfatase antibody|metachromatic leucodystrophy antibody|MGC125207 antibody|MLD antibody|OTTHUMP00000196546 antibody|OTTHUMP00000196548 antibody|TISP73 antibody

Gene ID: 410

UniProt: P15289