c-MYC antibody (AA 257-439)

Catalog No. ABIN3043598

Product Details anti-c-MYC Antibody

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Binding Specificity: AA 257-439

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Myc proto-oncogene protein(MYC) detection. Tested with WB in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Myc proto-oncogene protein(MYC) detection. Tested with WB in Human,Rat.
Gene Name: v-myc avian myelocytomatosis viral oncogene homolog
Protein Name: Myc proto-oncogene protein

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human c-Myc recombinant protein (Position: E257-A439). Human c-Myc shares 91% amino acid (aa) sequences identity with both mouse and rat c-Myc.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for c-Myc is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-c-MYC antibody (ABIN3043598)

Zhang, Chang, Zhao, Gao, Wang, Li, Zhang, Zhang, Xu: "The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180607, (2017) (PubMed).

Zhang, Chang, Li, Zhang, Xu: "The novel protein C3orf43 accelerates hepatocyte proliferation." in: Cellular & molecular biology letters, Vol. 22, pp. 21, (2017) (PubMed).

Hao, Wen, Li, Wang, Ni, Wang, Wang, Sun, Fan, Mao: "LiCl inhibits PRRSV infection by enhancing Wnt/?-catenin pathway and suppressing inflammatory responses." in: Antiviral research, Vol. 117, pp. 99-109, (2015) (PubMed).

Zhao, Li, Gao, Wang, Yan, Zhan, Liu, Mao, Chen, Wang et al.: "Design, synthesis and biological evaluation of N-alkyl or aryl substituted isoindigo derivatives as potential dual cyclin-dependent kinase 2 (CDK2)/glycogen synthase kinase 3? (GSK-3?) ..." in: European journal of medicinal chemistry, Vol. 86, pp. 165-74, (2014) (PubMed).

Wang, Gu, Wan, Zhu, Qiu, Weng, Yu, He: "Litopenaeus vannamei sterile-alpha and armadillo motif containing protein (LvSARM) is involved in regulation of Penaeidins and antilipopolysaccharide factors." in: PLoS ONE, Vol. 8, Issue 2, pp. e52088, (2013) (PubMed).

Li, Tian, Wang, Yao, Zhang, Lu, Sun, Gao, Xiao, Liu: "Down-regulation of miR-129-5p inhibits growth and induces apoptosis in laryngeal squamous cell carcinoma by targeting APC." in: PLoS ONE, Vol. 8, Issue 10, pp. e77829, (2013) (PubMed).

Yuan, Zhang, Cai, Ding, Wang, Chen, Wang, Yan, Lu: "Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer." in: Oncology reports, Vol. 29, Issue 6, pp. 2291-6, (2013) (PubMed).

Zhang, Lu, Yang, Li, Sun, Tao, Liu, Zhang, Ma: "HILI inhibits TGF-? signaling by interacting with Hsp90 and promoting T?R degradation." in: PLoS ONE, Vol. 7, Issue 7, pp. e41973, (2012) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Qin, Hua, Shuang, Wang, Cui: "Effects of matrine on HepG2 cell proliferation and expression of tumor relevant proteins in vitro." in: Pharmaceutical biology, Vol. 48, Issue 3, pp. 275-81, (2010) (PubMed).

Wang, Huo, Li, Fu, Xue, Yang, Wen, Ding, Duan: "Osteogenic role of endosomal chloride channels in MC3T3-E1 cells." in: Molecular and cellular biochemistry, Vol. 342, Issue 1-2, pp. 191-9, (2010) (PubMed).

Liu, Liu, Bai, Li, Yang: "Molecular cloning, expression and subcellular distribution of an alternative splice variant of the porcine Sirt2 gene." in: Molecular biology reports, Vol. 37, Issue 3, pp. 1671-6, (2010) (PubMed).

Long, Sun, Hu, Liang, Wang, Zhang, Cao, Zhen, Cui, Liu: "Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer." in: Oncology reports, Vol. 22, Issue 5, pp. 1189-95, (2009) (PubMed).

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: Myc Proto-Oncogene protein (MYC Products)

Synonyms: MRTL antibody, MYCC antibody, bHLHe39 antibody, c-Myc antibody, CMYC antibody, c-myc antibody, C-MYC antibody, LOC100136746 antibody, ARC antibody, B430311C09Rik antibody, MYC antibody, NOP antibody, Nop30 antibody, RNCMYC antibody, mMyc antibody, AU016757 antibody, Myc2 antibody, Niard antibody, Nird antibody, cmyc antibody, zc-myc antibody, c-myc II antibody, myc-B antibody, myc2 antibody, myc1 antibody, CMyc antibody, zgc:55680 antibody, CG10798 antibody, D-Myc antibody, DM antibody, DMYc antibody, Dm antibody, Dmel\CG10798 antibody, Dmyc antibody, EG:BACN5I9.1 antibody, anon-WO03040301.171 antibody, bHLHe57 antibody, c-MYC antibody, d-myc antibody, dMYC antibody, dMyc antibody, dMyc1 antibody, da antibody, dm antibody, dm/dMyc antibody, dm/myc antibody, dmyc antibody, dmyc1 antibody, l(1)G0139 antibody, l(1)G0354 antibody, l(1)G0359 antibody, myc antibody, MYC proto-oncogene, bHLH transcription factor antibody, v-myc myelocytomatosis viral oncogene homolog antibody, nucleolar protein 3 (apoptosis repressor with CARD domain) antibody, myelocytomatosis oncogene antibody, MYC proto-oncogene, bHLH transcription factor a antibody, MYC proto-oncogene, bHLH transcription factor L homeolog antibody, MYC proto-oncogene, bHLH transcription factor S homeolog antibody, v-myc myelocytomatosis viral oncogene homolog (avian) antibody, MYC proto-oncogene, bHLH transcription factor b antibody, CG10798 gene product from transcript CG10798-RB antibody, MYC antibody, LOC100136746 antibody, Nol3 antibody, Myc antibody, myca antibody, myc.L antibody, myc.S antibody, mycb antibody

Background: C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. C-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Synonyms: AU016757 antibody|Avian myelocytomatosis viral oncogene homolog antibody|bHLHe39 antibody|c Myc antibody|c Myc Epitope Tag antibody|c Myc Tag antibody|Class E basic helix-loop-helix protein 39 antibody|MRTL antibody|Myc antibody|Myc Epitope Tag antibody|Myc protein antibody|Myc proto oncogene protein antibody|Myc proto-oncogene protein antibody|myc-related translation/localization regulatory factor antibody|MYC_HUMAN antibody|Myc2 antibody|MYCC antibody|Myelocytomatosis oncogene antibody|Niard antibody|Nird antibody|Oncogene Myc antibody|OTTHUMP00000158589 antibody|Proto-oncogene c-Myc antibody|Protooncogene homologous to myelocytomatosis virus antibody|RNCMYC antibody|Transcription factor p64 antibody|Transcriptional regulator Myc-A antibodyV-Myc avian myelocytomatosis viral oncogene homolog antibody|v-myc myelocytomatosis viral oncogene homolog (avian) antibody

Gene ID: 4609

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect