ARID1A antibody (Middle Region)

Catalog No. ABIN3043527

This anti-ARID1A antibody is a Rabbit Polyclonal antibody detecting ARID1A in WB. Suitable for Human.

Quick Overview for ARID1A antibody (Middle Region) (ABIN3043527)

Target: ARID1A (AT Rich Interactive Domain 1A (SWI-Like) (ARID1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID1A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARID1A Antibody

Binding Specificity: AA 1021-1053, Middle Region

Purpose: Anti-ARID1A Antibody Picoband®

Sequence: KMWVDRYLAF TEEKAMGMTN LPAVGRKPLD LYR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ARID1A Antibody Picoband® (ABIN3043527). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ARID1A, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Dallas, P. B., Pacchione, S., Wilsker, D., Bowrin, V., Kobayashi, R., Moran, E. The human SWI-SNF complex protein p270 is an ARID family member with non-sequence-specific DNA binding activity. Molec. Cell. Biol. 20: 3137-3146, 2000. 2. Krosl, J., Mamo, A., Chagraoui, J., Wilhelm, B. T., Girard, S., Louis, I., Lessard, J., Perreault, C., Sauvageau, G. A mutant allele of the Swi/Snf member BAF250a determines the pool size of fetal liver hemopoietic stem cell populations. Blood 116: 1678-1684, 2010. 3. Nie, Z., Xue, Y., Yang, D., Zhou, S., Deroo, B. J., Archer, T. K., Wang, W. A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex. Molec. Cell. Biol. 20: 8879-8888, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ARID1A

Target: ARID1A (AT Rich Interactive Domain 1A (SWI-Like) (ARID1A))

Alternative Name: ARID1A

Background:

Synonyms: AT-rich interactive domain-containing protein 1A,ARID domain-containing protein 1A,B120,BRG1-associated factor 250,BAF250,BRG1-associated factor 250a,BAF250A,Osa homolog 1,hOSA1,SWI-like protein,SWI/SNF complex protein p270,SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1,hELD,ARID1A,BAF250, BAF250A, C1orf4, OSA1, SMARCF1,

Tissue Specificity: Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. .

Background: AT-rich interactive domain-containing protein 1A, also known as p270, is a protein that in humans is encoded by the ARID1A gene. This gene encodes a member of the SWI/SNF families, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is mapped to 1p36.11. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation.

Molecular Weight: 242 kDa

Gene ID: 8289

UniProt: O14497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Tube Formation