SIRT6 antibody (AA 14-180)

Catalog No. ABIN3043435

Product Details anti-SIRT6 Antibody

Target: SIRT6 (Sirtuin 6 (SIRT6))

Binding Specificity: AA 14-180

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-6(SIRT6) detection. Tested with WB, IHC-P in Human,Mouse.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-6(SIRT6) detection. Tested with WB, IHC-P in Human,Mouse.
Gene Name: sirtuin 6
Protein Name: NAD-dependent protein deacetylase sirtuin-6

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIRT6 recombinant protein (Position: D14-E180). Human SIRT6 shares 95% amino acid (aa) sequence identity with mouse SIRT6.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for SIRT6 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SIRT6

Target: SIRT6 (Sirtuin 6 (SIRT6))

Alternative Name: SIRT6 (SIRT6 Products)

Synonyms: 2810449N18Rik antibody, AI043036 antibody, Sir2l6 antibody, SIR2L6 antibody, zgc:86729 antibody, sirtuin 6 antibody, sirtuin 6 S homeolog antibody, Sirt6 antibody, SIRT6 antibody, sirt6.S antibody, sirt6 antibody

Background: SIRT6 is also known as SIR2L6. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.

Synonyms: 2810449N18Rik antibody|AI043036 antibody|Mono ADP ribosyltransferase sirtuin 6 antibody|Mono-ADP-ribosyltransferase sirtuin-6 antibody|NAD-dependent deacetylase sirtuin-6 antibody|NAD-dependent protein deacetylase sirtuin-6 antibody|Regulatory proteinSIR2 homolog antibody|SIR2 like 6 antibody|SIR2 like protein 6 antibody|Sir2 related protein type 6 antibody|SIR2-like protein 6 antibody|SIR2L6 antibody|SIRT 6 antibody|SIRT6 antibody|SIRT6_HUMAN antibody|Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) antibody|Sirtuin 6 antibody|Sirtuin type 6 antibody|Sirtuin6 antibody

Gene ID: 51548

UniProt: Q8N6T7