EIF4A2 antibody (N-Term)

Catalog No. ABIN3043426

Product Details anti-EIF4A2 Antibody

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Binding Specificity: AA 5-31, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Eukaryotic initiation factor 4A-II(EIF4A2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: SADYNREHGG PEGMDPDGVI ESNWNEI

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Eukaryotic initiation factor 4A-II(EIF4A2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: eukaryotic translation initiation factor 4A2
Protein Name: Eukaryotic initiation factor 4A-II

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human eIF4A2 (5-31aa SADYNREHGGPEGMDPDGVIESNWNEI), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: EIF4A2 (EIF4A2 Products)

Synonyms: BM-010 antibody, DDX2B antibody, EIF4A antibody, EIF4F antibody, eIF-4A-II antibody, eIF4A-II antibody, 4833432N07Rik antibody, Ddx2b antibody, Eif4 antibody, ddx2b antibody, eif4aii antibody, eif4f antibody, xeif4aii antibody, EIF4A2 antibody, EIF4AII antibody, wu:fd50g11 antibody, zgc:63783 antibody, eukaryotic translation initiation factor 4A2 antibody, eukaryotic translation initiation factor 4A2 L homeolog antibody, microRNA 1248 antibody, eukaryotic translation initiation factor 4A, isoform 2 antibody, Eif4a2 antibody, EIF4A2 antibody, eif4a2.L antibody, eif4a2 antibody, MIR1248 antibody

Background: Eukaryotic initiation factor 4A-II is a protein that in humans is encoded by the EIF4A2 gene. It is mapped to 18p11.2. Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice, yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation.

Synonyms: ATP dependent RNA helicase eIF4A 2 antibody|ATP-dependent RNA helicase eIF4A-2 antibody|BM-010 antibody|DDX2B antibody|eIF 4A II antibody|eIF-4A-II antibody|EIF4A antibody|eIF4A II antibody|eIF4A-II antibody|EIF4A2 antibody|EIF4F antibody|Eukaryotic initiation factor 4A II antibody|Eukaryotic initiation factor 4A-II antibody|eukaryotic translation initiation factor 4A isoform 2 antibody| Eukaryotic translation initiation factor 4A2 antibody|IF4A2_HUMAN antibody|N-terminally processed antibody

Gene ID: 1974

UniProt: Q14240