MAD1L1 antibody (AA 362-632)

Catalog No. ABIN3043387

Product Details anti-MAD1L1 Antibody

Target: MAD1L1 (MAD1 Mitotic Arrest Deficient-Like 1 (MAD1L1))

Binding Specificity: AA 362-632

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD1L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD1(MAD1L1) detection. Tested with WB, IHC-P in Human,Mouse,Rat

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD1(MAD1L1) detection. Tested with WB, IHC-P in Human,Mouse,Rat
Gene Name: MAD1 mitotic arrest deficient-like 1 (yeast)
Protein Name: Mitotic spindle assembly checkpoint protein MAD1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for MAD1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MAD1L1

Target: MAD1L1 (MAD1 Mitotic Arrest Deficient-Like 1 (MAD1L1))

Alternative Name: MAD1L1 (MAD1L1 Products)

Synonyms: MAD1 antibody, PIG9 antibody, TP53I9 antibody, TXBP181 antibody, AI173502 antibody, AW550425 antibody, Mad1 antibody, zgc:56571 antibody, MAD1-like protein 1 antibody, mitotic arrest deficient 1 like 1 antibody, MAD1 mitotic arrest deficient like 1 antibody, MAD1 mitotic arrest deficient 1-like 1 antibody, deafness, autosomal recessive 19 antibody, MAD1L1 antibody, Mad1l1 antibody, mad1l1 antibody, DFNB19 antibody

Background: Mitotic spindle assembly checkpoint protein MAD1 is a protein that in humans is encoded by the MAD1L1 gene. It is mapped to 7p22.3. MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 can function as a homodimer. It localizes to the centrosome during metaphase and to the spindle midzone and the midbody during anaphase and telophase. MAD1L1 may also play a role in cell cycle control and tumor suppression.

Synonyms: hMAD1 antibody|HsMAD1 antibody|MAD1 antibody|MAD1 mitotic arrest deficient like 1 (yeast) antibody|MAD1-like protein 1 antibody|MAD1L1 antibody|MD1L1_HUMAN antibody|Mitotic arrest deficient 1 antibody|Mitotic arrest deficient 1-like protein 1 antibody|Mitotic checkpoint MAD1 protein homolog antibody|Mitotic spindle assembly checkpoint protein MAD1 antibody|PIG9 antibody|Tax binding protein 181 antibody|Tax-binding protein 181 antibody|TP53I9 antibody|Tumor protein p53 inducible protein 9 antibody|TXBP181 antibody

Gene ID: 8379

UniProt: Q9Y6D9

Pathways: M Phase