PKM antibody (N-Term)

Catalog No. ABIN3043381

Product Details anti-PKM Antibody

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Binding Specificity: AA 475-500, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Pyruvate kinase PKM(PKM) detection. Tested with WB, IHC-P in Human.

Sequence: KDPVQEAWAE DVDLRVNFAM NVGKAR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Pyruvate kinase PKM(PKM) detection. Tested with WB, IHC-P in Human.
Gene Name: pyruvate kinase, muscle
Protein Name: Pyruvate kinase PKM

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2(475-500aa KDPVQEAWAEDVDLRVNFAMNVGKAR), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for PKM2 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM (PKM Products)

Synonyms: CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, CG7070 antibody, Dmel\\CG7070 antibody, PYK antibody, Pyk antibody, cb1029 antibody, fj33f03 antibody, pkm2 antibody, pkm2a antibody, wu:fj33f03 antibody, MGC53388 antibody, PKM antibody, PK-S antibody, pk3 antibody, pkm2b antibody, zgc:92037 antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, Pyruvate kinase antibody, pyruvate kinase M1/2a antibody, pyruvate kinase PKM antibody, pyruvate kinase (PK) antibody, pyruvate kinase antibody, pyruvate kinase M1/2 S homeolog antibody, pyruvate kinase M1/2b antibody, PKM antibody, Pkm antibody, PyK antibody, pkma antibody, pkm antibody, LOC710798 antibody, pyk antibody, pk antibody, LOC100534543 antibody, pkm.S antibody, pkmb antibody

Background: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.

Synonyms: CTHBP antibody|Cytosolic thyroid hormone binding protein antibody|Cytosolic thyroid hormone-binding protein antibody|KPYM_HUMAN antibody|MGC3932 antibody|OIP 3 antibody|OIP-3 antibody|Oip3 antibody|OIP3 antibody|OPA interacting protein 3 antibody|Opa-interacting protein 3 antibody|p58 antibody|PK Muscle type antibody|PK, muscle type antibody|PK2 antibody|Pk3 antibody|PKM antibody|PKM2 antibody|PYKM antibody|Pyruvate kinase 2/3 antibody|Pyruvate kinase 3 antibody|Pyruvate kinase isozymes M1/M2 antibody|Pyruvate kinase muscle antibody|Pyruvate kinase muscle isozyme antibody|Pyruvate kinase, muscle 2 antibody|TCB antibody|THBP1 antibody|Thyroid hormone binding protein 1 antibody|Thyroid hormone binding protein cytosolic antibody|Thyroid hormone-binding protein 1 antibody|Tumor M2 PK antibody|Tumor M2-PK antibody

Gene ID: 5315

UniProt: P14618

Pathways: Warburg Effect