PAPPA antibody (AA 95-388)

Catalog No. ABIN3043377

Product Details anti-PAPPA Antibody

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Binding Specificity: AA 95-388

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Pappalysin-1(PAPPA) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Pappalysin-1(PAPPA) detection. Tested with WB, IHC-P in Human.
Gene Name: pregnancy-associated plasma protein A, pappalysin 1
Protein Name: Pappalysin-1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PAPP A recombinant protein (Position: R95-Q388). Human PAPP A shares 88% amino acid (aa) sequence identity with mouse PAPP A.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for PAPP A is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPPA (PAPPA Products)

Synonyms: 8430414N03Rik antibody, IGFBP-4ase antibody, PAG1 antibody, PAPP-A antibody, ASBABP2 antibody, DIPLA1 antibody, PAPA antibody, PAPPA1 antibody, Pappa antibody, pappalysin 1 antibody, pregnancy-associated plasma protein A antibody, PAPPA antibody, Pappa antibody, Pappa1 antibody

Background: Pappalysin-1, also known as DIPLA1, is a protein that in humans is encoded by the PAPPA gene. It is mapped to 9q33.1. PAPPA is found in the ovarian follicles, follicular fluid, luteal cells, and fallopian tubes of nonpregnant women and in the seminal vesicles and seminal fluid of males. This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. It has been found that circulating PAPPA is a disulfide-bridged complex with proMBP in which the subunits of the constituents are present in a 1:1 Molar ratio.

Synonyms: ASBABP2 antibody|Aspecific BCL2 ARE binding protein 2 antibody|Differentially placenta 1 expressed protein antibody|DIPLA1 antibody|IGF dependent IGFBP 4 protease antibody|IGF-dependent IGFBP-4 protease antibody|IGFBP 4ase antibody|IGFBP-4ase antibody|IGFBP4ase antibody|Insulin like growth factor dependent IGF binding protein 4 protease antibody|Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody|PAPA antibody|PAPP A antibody|PAPP-A antibody|PAPP1_HUMAN antibody|PAPPA antibody|PAPPA1 antibody|Pappalysin 1 precursor antibody|Pappalysin-1 antibody|Pregnancy Associated Plasma Protein A antibody|Pregnancy associated plasma protein A pappalysin 1 antibody|Pregnancy-associated plasma protein A antibody

Gene ID: 5069

UniProt: Q13219