PKC eta antibody (AA 30-389)

Catalog No. ABIN3043361

Product Details anti-PKC eta Antibody

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Binding Specificity: AA 30-389

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC eta antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Protein kinase C eta type(PRKCH) detection. Tested with WB in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein kinase C eta type(PRKCH) detection. Tested with WB in Human.
Gene Name: protein kinase C, eta
Protein Name: Protein kinase C eta type

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PKC eta recombinant protein (Position: R30-D389). Human PKC eta shares 96.7% and 96.1% amino acid (aa) sequence identity with mouse and rat PKC eta, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-PKC eta antibody (ABIN3043361)

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Target Details for PKC eta

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Alternative Name: PRKCH (PRKCH Products)

Synonyms: PKC-L antibody, PKCL antibody, PRKCL antibody, nPKC-eta antibody, Pkch antibody, pkc-eta antibody, PRKCH antibody, prkch antibody, si:ch211-212g7.5 antibody, zgc:172124 antibody, protein kinase C eta antibody, protein kinase C, eta antibody, protein kinase C eta L homeolog antibody, protein kinase C, eta, b antibody, protein kinase C, eta, a antibody, protein kinase C epsilon antibody, PRKCH antibody, Prkch antibody, prkch.L antibody, prkchb antibody, prkcha antibody, prkch antibody, PRKCE antibody

Background: PRKCH is also known as PKC eta. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Also, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

Synonyms: KPCL_HUMAN antibody|MGC 5363 antibody|MGC26269 antibody|MGC5363 antibody|nPKC eta antibody| nPKC-eta antibody|PKC h antibody|PKC L antibody|PKC-L antibody|PKCh antibody|PKCL antibody| Prkch antibody|PRKCL antibody|Protein kinase C eta antibody|Protein kinase C eta type antibody

Gene ID: 5583

UniProt: P24723

Pathways: Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling