Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PKC delta antibody (AA 1-160)

This Rabbit Polyclonal antibody specifically detects PKC delta in WB, IF and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.
Catalog No. ABIN3043360

Quick Overview for PKC delta antibody (AA 1-160) (ABIN3043360)

Target

See all PKC delta (PKCd) Antibodies
PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reactivity

  • 197
  • 154
  • 142
  • 8
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Human

Host

  • 207
  • 10
Rabbit

Clonality

  • 193
  • 24
Polyclonal

Conjugate

  • 92
  • 13
  • 10
  • 9
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
This PKC delta antibody is un-conjugated

Application

  • 156
  • 81
  • 80
  • 70
  • 53
  • 49
  • 32
  • 24
  • 21
  • 20
  • 19
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Binding Specificity

    • 20
    • 19
    • 16
    • 15
    • 15
    • 15
    • 14
    • 8
    • 8
    • 8
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-160

    Purpose

    Anti-PKC delta/PRKCD Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins

    Characteristics

    Anti-PKC delta/PRKCD Antibody (ABIN3043360). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.

    Isotype

    IgG
  • Application Notes

    Western blot, 0.1-0.5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    1. Qu, Y., Misaghi, S., Izrael-Tomasevic, A., Newton, K., Gilmour, L. L., Lamkanfi, M., Louie, S., Kayagaki, N., Liu, J., Komuves, L., Cupp, J. E., Arnott, D., Monack, D., Dixit, V. M.Phosphorylation of NLRC4 is critical for inflammasome activation. Nature 490: 539-542, 2012. 2. Salzer, E., Santos-Valente, E., Klaver, S., Ban, S. A., Emminger, W., Prengemann, N. K., Garncarz, W., Mullauer, L., Kain, R., Boztug, H., Heitger, A., Arbeiter, K., Eitelberger, F., Seidel, M. G., Holter, W., Pollak, A., Pickl, W. F., Forster-Waldl, E., Boztug, K. B-cell deficiency and severe autoimmunity caused by deficiency of protein kinase C-delta. Blood 121: 3112-3116, 2013. 3. Tu, X., Joeng, K. S., Nakayama, K. I., Nakayama, K., Rajagopal, J., Carroll, T. J., McMahon, A. P., Long, F. Noncanonical Wnt signaling through G protein-linked PKC-delta activation promotes bone formation. Dev. Cell 12: 113-127, 2007.

    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

  • Target

    PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

    Alternative Name

    PRKCD

    Background

    Synonyms: Protein kinase C delta type,2.7.11.13,Tyrosine-protein kinase PRKCD,2.7.10.2,nPKC-delta,Protein kinase C delta type regulatory subunit,Protein kinase C delta type catalytic subunit,Sphingosine-dependent protein kinase-1,SDK1,PRKCD,

    Tissue Specificity: Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

    Background: Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved inB cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC). It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. What's more, it is also a part of a noncanonical WNT signaling cascade.

    Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

    Molecular Weight

    77 kDa

    Gene ID

    5580

    UniProt

    Q05655

    Pathways

    Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism
You are here:
Chat with us!