SIRT7 antibody (AA 8-350)

Catalog No. ABIN3043336

Product Details anti-SIRT7 Antibody

Target: SIRT7 (Sirtuin 7 (SIRT7))

Binding Specificity: AA 8-350

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-7(SIRT7) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-7(SIRT7) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: sirtuin 7
Protein Name: NAD-dependent protein deacetylase sirtuin-7

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIRT7 recombinant protein (Position: R8-G350). Human SIRT7 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat SIRT7, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for SIRT7 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SIRT7

Target: SIRT7 (Sirtuin 7 (SIRT7))

Alternative Name: SIRT7 (SIRT7 Products)

Synonyms: SIRT7 antibody, LOC796135 antibody, SIR2L7 antibody, sirtuin 7 antibody, phosphate cytidylyltransferase 2, ethanolamine antibody, SIRT7 antibody, sirt7 antibody, PCYT2 antibody, Sirt7 antibody

Background: SIRT7 is also known as SIR2L7. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Synonyms: NAD dependent deacetylase sirtuin 7 antibody|NAD dependent protein deacetylase sirtuin 7 antibody|NAD-dependent deacetylase sirtuin-7 antibody|Regulatory protein SIR2 homolog 7 antibody|Silent mating type information regulation 2 homolog antibody|Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody|SIR2 L7 antibody|SIR2 like protein 7 antibody|Sir2 related protein type 7 antibody|SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7 antibody|SIR2-like protein 7 antibody|SIR2L 7 antibody|SIR2L7 antibody|SIRT 7 antibody|SIRT7 antibody|SIRT7_HUMAN antibody|Sirtuin 7 antibody|Sirtuin type 7 antibody|Sirtuin7 antibody

Gene ID: 51547