S100A9 antibody (AA 2-114)

Catalog No. ABIN3043321

Product Details anti-S100A9 Antibody

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Binding Specificity: AA 2-114

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Protein S100-A9(S100A9) detection. Tested with WB, IHC-P, ELISA in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein S100-A9(S100A9) detection. Tested with WB, IHC-P, ELISA in Human.
Gene Name: S100 calcium binding protein A9
Protein Name: Protein S100-A9

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human S100A9 recombinant protein (Position: T2-P114). Human S100A9 shares 59.8% and 64.5% amino acid (aa) sequence identity with mouse and rat S100A9, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-S100A9 antibody (ABIN3043321)

Yan, Tian, Wang, Cheng, Xu, Song, Zhang, Zhang: "Age dependent changes in cartilage matrix, subchondral bone mass, and estradiol levels in blood serum, in naturally occurring osteoarthritis in Guinea pigs." in: International journal of molecular sciences, Vol. 15, Issue 8, pp. 13578-95, (2015) (PubMed).

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9 (S100A9 Products)

Background: S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. And S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase.

Synonyms: Leukocyte L1 complex heavy chain antibody|60B8AG antibody|AW546964 antibody|BEE22 antibody|CAGB antibody|Calgranulin B antibody| Calgranulin-B antibody|Calprotectin L1H subunit antibody|CFAG antibody|CGLB antibody|Cystic fibrosis antigen antibody|Cystic fibrosis antigen B antibody|HGNC:10499 antibody|L1AG antibody|Leukocyte L1 complex heavy chain antibody|LIAG antibody|MAC 387 antibody|MAC387 antibody|MIF antibody|Migration inhibitory factor related protein 14 antibody|Migration inhibitory factor-related protein 14 antibody| MRP 14 antibody|MRP 8 antibody|MRP-14 antibody|MRP14 antibody|Myeloid-related protein 14 antibody|NIF antibody| OTTHUMP00000015331 antibody|P14 antibody|Protein S100-A9 antibody|S100 A9 antibody|S100 calcium binding protein A9 antibody|S100 calcium binding protein A9 calgranulin B antibody|S100 calcium-binding protein A9 antibody|S100A9 antibody| S10A9_HUMAN antibody

Gene ID: 6280

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins