SRSF1 antibody (N-Term)

Catalog No. ABIN3043296

Product Details anti-SRSF1 Antibody

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Binding Specificity: AA 6-33, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Serine/arginine-rich splicing factor 1(SRSF1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: VIRGPAGNND CRIYVGNLPP DIRTKDIE

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Serine/arginine-rich splicing factor 1(SRSF1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: serine/arginine-rich splicing factor 1
Protein Name: Serine/arginine-rich splicing factor 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of Human SF2(6-33aa VIRGPAGNNDCRIYVGNLPPDIRTKDIE), identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat , The detection limit for SF2 is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SRSF1

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Alternative Name: SRSF1 (SRSF1 Products)

Synonyms: ASF antibody, SF2 antibody, SF2p33 antibody, SFRS1 antibody, SRp30a antibody, 1110054N12Rik antibody, 5730507C05Rik antibody, 6330415C05Rik antibody, AI482334 antibody, AW491331 antibody, Asf antibody, Sf2 antibody, Sfrs1 antibody, DKFZp469K2419 antibody, sfrs1 antibody, sfrs1b antibody, wu:fb80g05 antibody, zgc:111894 antibody, zgc:65898 antibody, zgc:76897 antibody, asf antibody, sf2 antibody, sf2p33 antibody, srp30a antibody, sfrs1a antibody, sfrs1l antibody, wu:fb52g10 antibody, wu:fb97g12 antibody, zgc:66146 antibody, serine and arginine rich splicing factor 1 antibody, serine/arginine-rich splicing factor 1 antibody, serine/arginine-rich splicing factor 1b antibody, serine/arginine-rich splicing factor 1 L homeolog antibody, serine/arginine-rich splicing factor 1a antibody, SRSF1 antibody, Srsf1 antibody, srsf1b antibody, srsf1.L antibody, srsf1a antibody

Background: SF2, also known as Serine/arginine-rich splicing factor 1 (SRSF1), is a protein that in humans is encoded by the SFRS1 gene. This gene encodes a member of the arginine/serine-rich splicing factor protein family. There is a pseudogene of this gene on chromosome 13. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. ASF/SF2 is necessary for all splicing reactions to occur, and influences splice site selection in a concentration-dependent manner, resulting in alternative splicing.

Synonyms: Alternative splicing factor 1 antibody|Alternative-splicing factor 1 antibody| arginine/serine-rich 1 antibody|ASF 1 antibody|ASF antibody|ASF-1 antibody|ASF1 antibody|FLJ53078 antibody|MGC5228 antibody|P33 subunit antibody|Pre mRNA splicing factor SF2 P33 subunit antibody|pre-mRNA-splicing factor SF2 antibody|Serine/arginine-rich splicing factor 1 antibody|SF2 antibody|SF2P33 antibody|SFRS1 antibody|Splicing factor 2 alternate splicing factor antibody|Splicing factor 2 antibody|Splicing factor antibody|Splicing factor arginine/serine rich 1 antibody|SR Splicing factor 1 antibody|SRp30a antibody|srsf1 antibody|SRSF1_HUMAN antibody

Gene ID: 6426

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization