ITGB1 antibody (AA 527-728)

Catalog No. ABIN3043259

Product Details anti-ITGB1 Antibody

Target: ITGB1 (Integrin beta 1 (ITGB1))

Binding Specificity: AA 527-728

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Integrin beta-1(ITGB1) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Integrin beta-1(ITGB1) detection. Tested with WB, IHC-P in Human.
Gene Name: integrin, beta 1
Protein Name: Integrin beta-1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ITGB1 recombinant protein (Position: N527-D728). Human ITGB1 shares 91% and 88% amino acid (aa) sequences identity with mouse and rat ITGB1, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for ITGB1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-ITGB1 antibody (ABIN3043259)

Tian, Tao, Zhao, Tai, Liu, Liu: "Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells." in: Experimental animals, Vol. 65, Issue 2, pp. 125-34, (2017) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." in: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Cheng, Gu, Yuan, Feng, Jia: "Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-α/ERK1/2 pathway: An in vitro investigation." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3709-10, (2016) (PubMed).

Yang, Sun, Geng, Ma, Sun, Fu: "Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1263-73, (2016) (PubMed).

Lin, Yang, Wang, Wang, Zhou, Liu, Peng: "Effect of mixed transplantation of autologous and allogeneic microskin grafts on wound healing in a rat model of acute skin defect." in: PLoS ONE, Vol. 9, Issue 1, pp. e85672, (2014) (PubMed).

Yin, Tian, Ye, Li, Wang, Cheng, Chen, Guo, Huang: "Nanog and ?-catenin: a new convergence point in EpSC proliferation and differentiation." in: International journal of molecular medicine, Vol. 29, Issue 4, pp. 587-92, (2012) (PubMed).

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1 (ITGB1 Products)

Background: Integrin beta-1, also known as CD29, is a protein that in humans is encoded by the ITGB1 gene. CD29 is an integrin unit associated with very late antigen receptors. It is known to conjoin with alpha-3 subunit to create alpha3beta1 complex that reacts to such molecules as netrin-1 and reelin. This gene contains a beta subunit that appears to be analogous to band-3 of integrin. It is mapped to 10p11.22. It is found that FER mediates crosstalk between CDH2 and CD29. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. The protein encoded by this gene is a beta subunit.

Synonyms: beta 1 subunit antibody|CD 29 antibody|CD29 antibody|CD29 antigen antibody|Fibrinogen Receptor beta subunit antibody|Fibronectin receptor subunit beta antibody|FNRB antibody|Glycoprotein IIa antibody|GP IIa antibody|GPIIA antibody|integrin beta 1 (fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12) antibody|Integrin beta-1 antibody|Integrin VLA 4 subunit beta antibody|integrin VLA-4 beta subunit antibody|Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody|ITB1_HUMAN antibody|ITGB 1 antibody|ITGB1 antibody|MDF2 antibody|MSK12 antibody|OTTHUMP00000019420 antibody|Very Late Activation Protein beta Polypeptide antibody|Very late activation protein, beta polypeptide antibody|VLA BETA antibody|VLA-4 subunit beta antibody|VLA-BETA antibody|VLAB antibody|VLAbeta antibody

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome