ITCH antibody (Middle Region)
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- Target See all ITCH Antibodies
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
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Binding Specificity
- AA 410-437, Middle Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ITCH antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purpose
- Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Itchy homolog(ITCH) detection. Tested with WB in Human.
- Sequence
- AMQQFNQRFI YGNQDLFATS QSKEFDPL
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Itchy homolog(ITCH) detection. Tested with WB in Human.
Gene Name: itchy E3 ubiquitin protein ligase
Protein Name: E3 ubiquitin-protein ligase Itchy homolog - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human ITCH/AIP4 (410-437aa AMQQFNQRFIYGNQDLFATSQSKEFDPL), different from the related mouse sequence by one amino acid.
- Isotype
- IgG
- Top Product
- Discover our top product ITCH Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- Alternative Name
- ITCH (ITCH Products)
- Synonyms
- AIF4 antibody, AIP4 antibody, NAPP1 antibody, dJ468O1.1 antibody, 6720481N21Rik antibody, 8030492O04Rik antibody, A130065M08 antibody, C230047C07Rik antibody, itchy E3 ubiquitin protein ligase antibody, itchy, E3 ubiquitin protein ligase antibody, ITCH antibody, Itch antibody
- Background
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ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Synonyms: AIF4 antibody|AIP4 antibody|Atrophin 1 interacting protein 4 antibody|Atrophin-1-interacting protein 4 antibody|dJ468O1.1 antibody|dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) antibody|dJ468O1.1 atrophin 1 interacting protein 4 AIP4 antibody|E3 ubiquitin protein ligase Itchy homolog antibody|E3 ubiquitin-protein ligase Itchy homolog antibody|EC 6.3.2 antibody|Itch antibody|ITCH_HUMAN antibody|Itchy E3 ubiquitin protein ligase antibody|Itchy E3 ubiquitin protein ligase homolog antibody|Itchy E3 ubiquitin protein ligase homolog mouse antibody|Itchy E3 ubiquitin protein ligase, mouse, homolog of antibody|itchy homolog E3 ubiquitin protein ligase antibody|Itchy mouse homolog E3 ubiquitin protein ligase antibody|NAPP1 antibody|NFE2 associated polypeptide 1 antibody| NFE2-associated polypeptide 1 antibody|Ubiquitin protein ligase ITCH antibody - Gene ID
- 83737
- UniProt
- Q96J02
- Pathways
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
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