Annexin IV antibody (N-Term)

Catalog No. ABIN3043239

Product Details anti-Annexin IV Antibody

Target: Annexin IV (ANXA4) (Annexin A4 (ANXA4))

Binding Specificity: AA 7-21, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Annexin A4(ANXA4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: TVKAASGFNA MEDAQ

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Annexin A4(ANXA4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: annexin A4
Protein Name: Annexin A4

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin IV(7-21aa TVKAASGFNAMEDAQ), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for ANXA4 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Annexin IV antibody (ABIN3043239)

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." in: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Target Details for Annexin IV

Target: Annexin IV (ANXA4) (Annexin A4 (ANXA4))

Alternative Name: ANXA4 (ANXA4 Products)

Synonyms: ANXA4 antibody, Anx4 antibody, XAnx4 antibody, pig28 antibody, X-anx4 antibody, Xanx-4 antibody, ANXIV antibody, anx4 antibody, ANX4 antibody, PIG28 antibody, ZAP36 antibody, Annexin-4 antibody, P32.5 antibody, PAP-II antibody, PP4-X antibody, AI265406 antibody, AIV antibody, AW106930 antibody, annexin A4 antibody, annexin A4 L homeolog antibody, ANXA4 antibody, anxa4 antibody, CC1G_00713 antibody, LOC100280951 antibody, Anxa4 antibody, anxa4.L antibody

Background: Annexin IV, also known as placental anticoagulant protein II, or zymogen granule membrane-associated protein, 36-KD(ZAP36), is one of the family of calcium-dependent phospholipid-binding proteins whose normal function is uncertain. Annexin IV has 45 to 59 % identity with other members of the annexin family. Annexin IV gene spans 18 to 56 kb. And it is located on mouse chromosome 6 in a region of conserved synteny with human chromosome 2. Rat annexin IV is ubiquitously expressed in epithelial tissues. Zap36 associated with zymogen granule membranes in dog and rat pancreas. Human annexin IV gene is mapped to chromosome 2p13.

Synonyms: 35 beta calcimedin antibody|35-beta calcimedin antibody|AIV antibody|Annexin 4 antibody|Annexin A4 antibody|Annexin IV(placental anticoagulant protein II) antibody|Annexin IV antibody|Annexin IV placental anticoagulant protein II antibody|Annexin-4 antibody|AnnexinA4 antibody|AnnexinIV antibody|ANX 4 antibody|ANX A4 antibody|ANX4 antibody|ANXA 4 antibody|ANXA4 antibody|ANXA4 protein antibody|ANXA4_HUMAN antibody|Carbohydrate binding protein P33/P41 antibody|Carbohydrate-binding protein p33/p41 antibody|Chromobindin 4 antibody|Chromobindin-4 antibody|Chromobindin4 antibody|DKFZp686H02120 antibody|Endonexin I antibody|EndonexinI antibody|Lipocortin IV antibody|LipocortinIV antibody|MGC75105 antibody|OTTHUMP00000160052 antibody|OTTHUMP00000202207 antibody|P32.5 antibody|P33/41 antibody|PAP II antibody|PAP-II antibody|PAPII antibody|PIG 28 antibody|PIG28 antibody|Placental anticoagulant protein II antibody|PP4 X antibody|PP4-X antibody|PP4X antibody|Proliferation inducing gene 28 antibody|Proliferation inducing protein 28 antibody|Protein II antibody|Xanx-4 antibody|ZAP 36 antibody|ZAP36 antibody

UniProt: P09525