Sonic Hedgehog antibody (N-Term)

Catalog No. ABIN3043230

Product Details anti-Sonic Hedgehog Antibody

Target: Sonic Hedgehog (SHH)

Binding Specificity: AA 203-218, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Sonic hedgehog protein(SHH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: ATVHLEQGGT KLVKDL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Sonic hedgehog protein(SHH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: sonic hedgehog
Protein Name: Sonic hedgehog protein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog(203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Sonic Hedgehog antibody (ABIN3043230)

Dai, Ai, Du, Chen: "Sonic hedgehog expression correlates with distant metastasis in pancreatic adenocarcinoma." in: Pancreas, Vol. 40, Issue 2, pp. 233-6, (2011) (PubMed).

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: SHH (SHH Products)

Synonyms: HHG1 antibody, HLP3 antibody, HPE3 antibody, MCOPCB5 antibody, SMMCI antibody, TPT antibody, TPTPS antibody, 9530036O11Rik antibody, Dsh antibody, Hhg1 antibody, Hx antibody, Hxl3 antibody, M100081 antibody, fc83d08 antibody, shh antibody, syu antibody, vhh-1 antibody, vhh1 antibody, wu:fc83d08 antibody, Xhh antibody, hedgehog antibody, xshh antibody, SHH antibody, twh antibody, twhh antibody, sonic hedgehog antibody, sonic hedgehog a antibody, sonic hedgehog L homeolog antibody, sonic hedgehog protein A antibody, sonic hedgehog b antibody, SHH antibody, Shh antibody, shha antibody, shh.L antibody, shh antibody, shhb antibody

Background: The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.

Synonyms: HHG 1 antibody|HHG-1 antibody|HHG1 antibody|HLP 3 antibody|HLP3 antibody|Holoprosencephaly 3 antibody|HPE 3 antibody|HPE3 antibody|MCOPCB5 antibody|SHH antibody|SHH_HUMAN antibody|SMMC I antibody|SMMCI antibody|Sonic Hedgehog(Drosophila) homolog antibody|sonic hedgehog homolog(Drosophila) antibody|Sonic hedgehog homolog antibody|Sonic hedgehog protein antibody|Sonic hedgehog protein C-product antibody|TPT antibody|TPTPS antibody

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development