Surfactant Protein A1 antibody (C-Term)

Catalog No. ABIN3043205

Product Details anti-Surfactant Protein A1 Antibody

Target: Surfactant Protein A1 (SFTPA1)

Binding Specificity: AA 233-248, C-Term

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Surfactant Protein A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Pulmonary surfactant-associated protein A(SFTPA1) detection. Tested with WB, IHC-P in Mouse,Rat.

Sequence: WNDKGCLQYR LAICEF

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Pulmonary surfactant-associated protein A(SFTPA1) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: surfactant protein A1
Protein Name: Pulmonary surfactant-associated protein A

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse SFTPA1(233-248aa WNDKGCLQYRLAICEF), identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Surfactant Protein A1 antibody (ABIN3043205)

Ostermann, Seeliger, David, Flasche, Maus, Reinboth, Christmann, Neumann, Brand, Seltmann, Bühling, Paton, Roth, Vogl, Viemann, Welte, Maus: "S100A9 is indispensable for survival of pneumococcal pneumonia in mice." in: PLoS pathogens, Vol. 19, Issue 7, pp. e1011493, (2023) (PubMed).

Ostermann, Maus, Stolper, Schütte, Katsarou, Tumpara, Pich, Mueller, Janciauskiene, Welte, Maus: "Alpha-1 antitrypsin deficiency impairs lung antibacterial immunity in mice." in: JCI insight, Vol. 6, Issue 3, (2021) (PubMed).

Hu, Wang, Rao, Zhao, Yang, Hu, He, Xia, Liu, Zhen, Di, Xie, Xia, Zhu: "Alterations in the endometrium of rats, rabbits, and Macaca mulatta that received an implantation of copper/low-density polyethylene nanocomposite." in: International journal of nanomedicine, Vol. 9, pp. 1127-38, (2015) (PubMed).

Zhou, Chen, Jiang, Feng, Han: "Effects of bone marrow-derived mesenchymal stem cells transfected with survivin on pulmonary fibrosis in mice." in: Experimental and therapeutic medicine, Vol. 10, Issue 5, pp. 1857-1864, (2015) (PubMed).

Wu, You, Ma, Li, Yuan, Li, Ye, Liu, Yao, Chen, Lai, Yang: "Role of transient receptor potential ion channels and evoked levels of neuropeptides in a formaldehyde-induced model of asthma in BALB/c mice." in: PLoS ONE, Vol. 8, Issue 5, pp. e62827, (2013) (PubMed).

Target Details for Surfactant Protein A1

Target: Surfactant Protein A1 (SFTPA1)

Alternative Name: SFTPA1 (SFTPA1 Products)

Background: Surfactant protein A is an innate immune system collectin. The gene encoding SP-A map to mouse chromosome 14. Surfactant protein-A, which plays a role in innate host defense in the lung, is also expressed in the Eustachian tube. We report that the frequency of specific surfactant protein-A haplotypes and genotypes differs between children with recurrent otitis media compared with a control population.

Synonyms: 35 kDa pulmonary surfactant-associated protein antibody|Alveolar proteinosis protein antibody|COLEC4 antibody|Collectin 4 antibody|PSAP antibody|PSPA antibody|Pulmonary surfactant apoprotein antibody|Pulmonary surfactant-associated protein A1 antibody|SFTP1 antibody|SFTPA antibody|SFTPA1 antibody|SFTPA1B antibody|SP A antibody|SP A1 antibody

UniProt: P35242