AMY1A antibody (N-Term)

Catalog No. ABIN3043188

Product Details anti-AMY1A Antibody

Target: AMY1A (Amylase, alpha 1A (Salivary) (AMY1A))

Binding Specificity: AA 30-44, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMY1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Alpha-amylase 1(AMY1A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: HLFEWRWVDI ALECE

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Alpha-amylase 1(AMY1A) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: amylase, alpha 1A(salivary)
Protein Name: Alpha-amylase 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1(30-44aa HLFEWRWVDIALECE), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AMY1A

Target: AMY1A (Amylase, alpha 1A (Salivary) (AMY1A))

Alternative Name: AMY1A (AMY1A Products)

Synonyms: Amy1 antibody, AMY1A antibody, AMY1 antibody, amylase, alpha 1A antibody, amylase, alpha 1A (salivary) antibody, alpha-amylase 1 antibody, pancreatic alpha-amylase antibody, Amy1a antibody, AMY1A antibody, LOC100446394 antibody, LOC100593892 antibody

Background: Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it begins the chemical process of digestion. By in situ hybridization combined with high resolution cytogenetics, the amylase gene is mapped to 1p21.Amylase enzymes find use in bread making and to break down complex sugars such as starch(found in flour) into simple sugars. Yeast then feeds on these simple sugars and converts it into the waste products of alcohol and CO2.

Synonyms: 1 antibody|4-alpha-D-glucan glucanohydrolase 1 antibody|Alpha-amylase 1 antibody|AMY1_HUMAN antibody|AMY1A antibody|AMY1B antibody|AMY1C antibody|Salivary alpha-amylase antibody

UniProt: P04745