Kallikrein 5 antibody (C-Term)

Catalog No. ABIN3043138

Product Details anti-Kallikrein 5 Antibody

Target: Kallikrein 5 (KLK5)

Binding Specificity: AA 278-293, C-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kallikrein 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Kallikrein 1-related peptidase b5(KLK5) detection. Tested with WB, IHC-P in Mouse,Rat.

Sequence: NLCEFVKWIK DTMNSN

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Kallikrein 1-related peptidase b5(KLK5) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: kallikrein-related peptidase 5
Protein Name: Kallikrein 1-related peptidase b5

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Kallikrein 5(278-293aa NLCEFVKWIKDTMNSN), different from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Kallikrein 5

Target: Kallikrein 5 (KLK5)

Alternative Name: KLK5 (KLK5 Products)

Synonyms: KLK-L2 antibody, KLKL2 antibody, SCTE antibody, 1110030O19Rik antibody, KAL antibody, KALA antibody, Klk1 antibody, Klk5 antibody, RATKALA antibody, kallikrein related peptidase 5 antibody, kallikrein related-peptidase 5 antibody, kallikrein 5-like antibody, KLK5 antibody, Klk5 antibody, Klk5l antibody

Background: KLK5(Kallikrein-related peptidase 5), formerly known as stratum corneum tryptic enzyme(SCTE) or KLKL2, is a serine protease expressed in the epidermis. The KLK5 gene is one of the fifteen kallikrein subfamily members which is located on 19q13.41. KLK5 has been suggested to regulate cell shedding in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. The KLK5 gene contains 5 coding exons spanning 9.3. KLK5 expression is upregulated in a breast cancer cell line in the presence of estrogens and progestins and is associated with more aggressive forms of epithelial ovarian carcinoma.

Synonyms: HK5 antibody|Kallikrein 5 antibody|Kallikrein L2 antibody|Kallikrein Like 2 antibody|Kallikrein like protein 2 antibody|Kallikrein related peptidase 5 antibody|Kallikrein-5 antibody|Kallikrein-like protein 2 antibody|Kallikrein5 antibody|KLK 5 antibody|KLK L2 antibody|KLK-L2 antibody|KLK5 antibody|KLK5_HUMAN antibody|KLKL 2 antibody|KLKL2 antibody|SCTE antibody|Stratum Corneum Tryptic Enzyme antibody|UNQ570/PRO1132 antibody

UniProt: Q9D140

Pathways: Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling