Neurotrophin 3 antibody (Middle Region)

Catalog No. ABIN3043024

Product Details anti-Neurotrophin 3 Antibody

Target: Neurotrophin 3 (NTF3)

Binding Specificity: AA 174-189, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurotrophin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Neurotrophin-3(NTF3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: TVLGEIKTGN SPVKQY

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Neurotrophin-3(NTF3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: neurotrophin 3
Protein Name: Neurotrophin-3(NT-3)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Neurotrophin 3(174-189aa TVLGEIKTGNSPVKQY), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Neurotrophin 3 antibody (ABIN3043024)

Guo, Zeng, Li, Huang, Liu, Li, Ding, Wu, Cai: "Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury." in: Spinal cord, Vol. 45, Issue 1, pp. 15-24, (2007) (PubMed).

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: NTF3 (NTF3 Products)

Synonyms: HDNF antibody, NGF-2 antibody, NGF2 antibody, NT3 antibody, NT-3 antibody, wu:fl98d04 antibody, hdnf antibody, ngf-2 antibody, ngf2 antibody, nt-3 antibody, nt3 antibody, NTF3 antibody, AI316846 antibody, AI835689 antibody, Nt3 antibody, Ntf-3 antibody, neurotrophin 3 antibody, neurotrophin 3 S homeolog antibody, neurotrophin 3 L homeolog antibody, NTF3 antibody, ntf3 antibody, ntf3.S antibody, Ntf3 antibody, ntf3.L antibody

Background: Neurotrophin-3 is a member of a family of neurotrophic factors, that is closely related to both nerve growth factor and brain derived neurotrophic factor. These proteins are involved in the maintenance of the adult nervous system and affect development of neurons in the embryo when it is expressed in human placenta. NT3 deficient mice generated by gene targeting display severe movement defects of the limbs.

Synonyms: HDNF antibody|MGC129711 antibody|Nerve growth factor 2 antibody|Neurotrophic factor antibody|Neurotrophin 3 precursor antibody|Neurotrophin-3 antibody|Neurotrophin3 antibody|NGF 2 antibody|NGF-2 antibody|NGF2 antibody|NT 3 antibody|NT-3 antibody|NT3 antibody|NTF 3 antibody|NTF3 antibody|NTF3_HUMAN antibody

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway