HMGB4 antibody (N-Term)

Catalog No. ABIN3043021

Product Details anti-HMGB4 Antibody

Target: HMGB4 (High Mobility Group Box 4 (HMGB4))

Binding Specificity: AA 24-41, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB4 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for High mobility group protein B4(HMGB4) detection. Tested with WB in Human,Mouse,Rat.

Sequence: RNKFKEQQPN TYVGFKEF

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for High mobility group protein B4(HMGB4) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: high mobility group box 4
Protein Name: High mobility group protein B4

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB4(24-41aa RNKFKEQQPNTYVGFKEF), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-HMGB4 antibody (ABIN3043021)

Rouhiainen, Zhao, Vanttola, Qian, Kulesskiy, Kuja-Panula, Gransalke, Grönholm, Unni, Meistrich, Tian, Auvinen, Rauvala: "HMGB4 is expressed by neuronal cells and affects the expression of genes involved in neural differentiation." in: Scientific reports, Vol. 6, pp. 32960, (2018) (PubMed).

Zhao, Xue, Wang: "HMGB1, TGF-β and NF-κB are associated with chronic allograft nephropathy." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 6138-6146, (2017) (PubMed).

Target Details for HMGB4

Target: HMGB4 (High Mobility Group Box 4 (HMGB4))

Alternative Name: HMGB4 (HMGB4 Products)

Synonyms: HMGB4 antibody, dJ1007G16.5 antibody, 1700001F22Rik antibody, AI326135 antibody, high mobility group box 4 antibody, high-mobility group box 4 antibody, HMGB4 antibody, Hmgb4 antibody

Background: HMGB4 is HMG2 like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, which is found in a variety of eukaryotic chromosomal proteins and transcription. HMGB proteins are phosphorylated to various extents reveals that the existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.

Synonyms: High mobility group box 4 antibody|High mobility group protein B4 antibody|HMG2 like antibody