T-Bet antibody (C-Term)

Catalog No. ABIN3042957

Product Details anti-T-Bet Antibody

Target: T-Bet

Binding Specificity: AA 522-535, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for T-box transcription factor TBX21(TBX21) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DKEAEGQFYN YFPN

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for T-box transcription factor TBX21(TBX21) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: T-box 21
Protein Name: T-box transcription factor TBX21

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet(522-535aa DKEAEGQFYNYFPN), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for TBX21 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-T-Bet antibody (ABIN3042957)

Miao, Run-Ming, Yi: "T-Bet Mediated Anti-Neoplastic Effects of Dendritic Cell-Cytokine Induced Killer Cells in vitro." in: Iranian journal of pediatrics, Vol. 22, Issue 1, pp. 43-51, (2012) (PubMed).

Target Details for T-Bet

Target: T-Bet

Alternative Name: TBX21 (T-Bet Products)

Synonyms: T-PET antibody, T-bet antibody, TBET antibody, TBLYM antibody, TBT1 antibody, Tbet antibody, Tblym antibody, T-box 21 antibody, TBX21 antibody, Tbx21 antibody

Background: TBX21(T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang(2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al.(2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer(NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al.(2002) observed reduced expression of the T(H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.

Synonyms: T bet antibody|T box 21 antibody|T box expressed in T cells antibody|T box protein 21 antibody|T box transcription factor TBX21 antibody|T cell specific T box transcription factor antibody|T cell specific T box transcription factor T bet antibody|T PET antibody|T-box protein 21 antibody|T-box transcription factor TBX21 antibody|T-cell-specific T-box transcription factor T-bet antibody|TBET antibody|TBLYM antibody|TBX 21 antibody|TBX21 antibody|TBX21_HUMAN antibody|TPET antibody|Transcription factor TBLYM antibody