SPARCL1 antibody (C-Term)

Catalog No. ABIN3042909

Product Details anti-SPARCL1 Antibody

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Binding Specificity: AA 649-664, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARCL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for SPARC-like protein 1(SPARCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: HCFGIKEEDI DENLLF

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for SPARC-like protein 1(SPARCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: SPARC-like 1(hevin)
Protein Name: SPARC-like protein 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1(649-664aa HCFGIKEEDIDENLLF), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SPARCL1

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Alternative Name: SPARCL1 (SPARCL1 Products)

Synonyms: LOC445896 antibody, SPARCL1 antibody, sparcl antibody, sparcl1 antibody, DKFZp459I2327 antibody, Ecm2 antibody, Sc1 antibody, hevin antibody, mast9 antibody, PIG33 antibody, SC1 antibody, SPARCL-1 antibody, SPARCL1 antibody, SPARC like 1 antibody, SPARC-like 1 antibody, SPARC-like protein 1 antibody, LOC445896 antibody, SPARCL1 antibody, sparcl1 antibody, Sparcl1 antibody

Background: SPARCL1(SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules(HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. Hevin is highly expressed in HEV and is though to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration(Girard and Springer, 1995).

Synonyms: Ecm2 antibody|Extracellular matrix protein 2 antibody|Hevin antibody|High endothelial venule protein antibody|MAST 9 antibody|mast9 antibody|Matrix glycoprotein Sc1 antibody|PIG33 antibody|proliferation inducing protein 33 antibody|RAGS1 antigen antibody|SC1 antibody|SPARC like 1 antibody|SPARC-like protein 1 antibody|Sparcl1 antibody|SPRL1_HUMAN antibody

UniProt: Q14515