SDC3 antibody (N-Term)

Catalog No. ABIN3042886

Product Details anti-SDC3 Antibody

Target: SDC3 (Syndecan 3 (SDC3))

Binding Specificity: AA 45-60, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SDC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Syndecan-3(SDC3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: AQRWRNENFE RPVDLE

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Syndecan-3(SDC3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: syndecan 3
Protein Name: Syndecan-3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse Syndecan 3/SDC3 (45-60aa AQRWRNENFERPVDLE), identical to the related rat sequence, and different from the related human sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-SDC3 antibody (ABIN3042886)

Yao, Zhang, Huang, Li, Gao: "Pleiotrophin and N-syndecan promote perineural invasion and tumor progression in an orthotopic mouse model of pancreatic cancer." in: World journal of gastroenterology, Vol. 23, Issue 21, pp. 3907-3914, (2017) (PubMed).

Yao, Li, Li, Feng, Gao: "Midkine promotes perineural invasion in human pancreatic cancer." in: World journal of gastroenterology, Vol. 20, Issue 11, pp. 3018-24, (2014) (PubMed).

Target Details for SDC3

Target: SDC3 (Syndecan 3 (SDC3))

Alternative Name: SDC3 (SDC3 Products)

Synonyms: wu:fa97a01 antibody, SDC3 antibody, sdcn antibody, synd3 antibody, Xsyn-3 antibody, N-syndecan antibody, SDCN antibody, SYND3 antibody, Synd3 antibody, mKIAA0468 antibody, syn-3 antibody, syndecan 3 antibody, sdc3 antibody, SDC3 antibody, Sdc3 antibody

Background: Syndecan-3, also called SYND3 is a protein that in humans is encoded by the SDC3 gene. This gene was assigned to human chromosome 1p35.2. This gene can function as in trans HIV receptors via binding of HIV-1 gp120 to the syndecan heparin sulfate chains. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, and possibly by transferring signals from the cell surface in a sugar-dependent mechanism. In wildtype mice, SDC3 was expressed in hypothalamic regions that control energy balance. It was proposed that oscillation of hypothalamic SDC3 levels physiologically modulates feeding behavior.

Synonyms: N-syndecan antibody|Sdc3 antibody|SDC3_HUMAN antibody|SDCN antibody|SYND3 antibody|syndecan proteoglycan 3 antibody|Syndecan-3 antibody

UniProt: Q64519

Pathways: Glycosaminoglycan Metabolic Process