E2F3 antibody (C-Term)

Catalog No. ABIN3042877

Product Details anti-E2F3 Antibody

Target: E2F3

Binding Specificity: AA 446-465, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Transcription factor E2F3(E2F3) detection. Tested with WB in Human,Mouse.

Sequence: DLFDAYDLEK LPLVEDFMCS

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Transcription factor E2F3(E2F3) detection. Tested with WB in Human,Mouse.
Gene Name: E2F transcription factor 3
Protein Name: Transcription factor E2F3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3(446-465aa DLFDAYDLEKLPLVEDFMCS), identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-E2F3 antibody (ABIN3042877)

Xie, Li, Wang, Jiao, Zhang, Zhang: "miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells." in: Oncology reports, Vol. 23, Issue 6, pp. 1693-700, (2010) (PubMed).

Wang, Li, Zhang, Li, Yue, Xie, Xie: "Regulating A549 cells growth by ASO inhibiting miRNA expression." in: Molecular and cellular biochemistry, Vol. 339, Issue 1-2, pp. 163-71, (2010) (PubMed).

Target Details for E2F3

Target: E2F3

Abstract: E2F3 Products

Synonyms: E2F-3 antibody, E2F3b antibody, E2f3a antibody, mKIAA0075 antibody, RGD1561600 antibody, E2F transcription factor 3 antibody, E2F transcription factor 3 S homeolog antibody, E2F3 antibody, e2f3.S antibody, E2f3 antibody

Background: Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein(pRB) to regulate the expression of genes involved in the cell cycle.

Synonyms: DKFZp686C18211 antibody|E2F 3 antibody|E2F transcription factor 3 antibody|E2F-3 antibody|E2F3 antibody|E2F3_HUMAN antibody|KIAA0075 antibody|MGC104598 antibody|Transcription factor E2F 3 antibody|Transcription factor E2F3 antibody

UniProt: O00716