SLC1A1 antibody (N-Term)

Catalog No. ABIN3042859

This anti-SLC1A1 antibody is a Rabbit Polyclonal antibody detecting SLC1A1 in WB. Suitable for Human.

Quick Overview for SLC1A1 antibody (N-Term) (ABIN3042859)

Target: SLC1A1 (Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SLC1A1 Antibody

Binding Specificity: AA 10-27, N-Term

Purpose: Anti-EAAT3/SLC1A1 Antibody Picoband®

Sequence: EWKRFLKNNW VLLSTVAA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-EAAT3/SLC1A1 Antibody (ABIN3042859). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human EAAT3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Berman, A. E., Chan, W. Y., Brennan, A. M., Reyes, R. C., Adler, B. L., Suh, S. W., Kauppinen, T. M., Edling, Y., Swanson, R. A. N-acetylcysteine prevents loss of dopaminergic neurons in the EAAC1-/- mouse. Ann. Neurol. 69: 509-520, 2011. 2. Myles-Worsley, M., Tiobech, J., Browning, S. R., Korn, J., Goodman, S., Gentile, K., Melhem, N., Byerley, W., Faraone, S. V., Middleton, F. A. Deletion at the SLC1A1 glutamate transporter gene co-segregates with schizophrenia and bipolar schizoaffective disorder in a 5-generation family. Am. J. Med. Genet. 162B: 87-95, 2013.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SLC1A1

Target: SLC1A1 (Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))

Alternative Name: SLC1A1

Background:

Synonyms: Excitatory amino acid transporter 3,Excitatory amino-acid carrier 1,Neuronal and epithelial glutamate transporter,Sodium-dependent glutamate/aspartate transporter 3,Solute carrier family 1 member 1,SLC1A1,EAAC1, EAAT3,

Tissue Specificity: Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). .

Background: Solute carrier family 1 member 1, also called SLC1A1, is a protein that in humans is encoded by the SLC1A1 gene. By Southern analysis of a panel of human/rodent somatic cell hybrids and by fluorescence in situ hybridization (FISH), this gene is mapped to 9p24.2. This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are though to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect.

Molecular Weight: 15 kDa

UniProt: P43005

Pathways: Dicarboxylic Acid Transport