Cathepsin B antibody (AA 80-333)

Catalog No. ABIN3042773

Product Details anti-Cathepsin B Antibody

Target: Cathepsin B (CTSB)

Binding Specificity: AA 80-333

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin B antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Cathepsin B(CTSB) detection. Tested with WB in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cathepsin B(CTSB) detection. Tested with WB in Human.
Gene Name: cathepsin B
Protein Name: Cathepsin B

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cathepsin B recombinant protein (Position: L80-D333). Human Cathepsin B shares 83% and 84% amino acid (aa) sequences identity with mouse and rat Cathepsin B, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Cathepsin B is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Cathepsin B antibody (ABIN3042773)

Zheng, Li, Wang, Wang, Liao, Hu, Fan, Kang, Zeng, Wu, Wu, Zhang, Wang, He et al.: "Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage ..." in: Autophagy, Vol. 12, Issue 9, pp. 1593-613, (2017) (PubMed).

Zhang, Mai, Yang, Dong, Zheng, Yang: "CTSB promotes porcine preadipocytes differentiation by degrading fibronectin and attenuating the Wnt/β-catenin signaling pathway." in: Molecular and cellular biochemistry, Vol. 395, Issue 1-2, pp. 53-64, (2014) (PubMed).

Yang, Lü, Hong, Xi, Zhang: "The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function." in: Biomaterials, Vol. 35, Issue 24, pp. 6195-205, (2014) (PubMed).

Target Details for Cathepsin B

Target: Cathepsin B (CTSB)

Alternative Name: CTSB (CTSB Products)

Synonyms: APPS antibody, CPSB antibody, CB antibody, apps antibody, cpsb antibody, LOC692390 antibody, CTSB antibody, cathepsin B antibody, cathepsin B S homeolog antibody, CTSB antibody, Ctsb antibody, ctsb.S antibody, LOC692390 antibody

Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. And this gene is mapped to chromosome 8p22. The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Synonyms: Amyloid precursor protein secretase antibody|APP secretase antibody|APPS antibody|CATB_HUMAN antibody|Cathepsin B antibody|Cathepsin B heavy chain antibody|Cathepsin B1 antibody|CathepsinB antibody|CPSB antibody|CTSB antibody|Cysteine protease antibody|OTTHUMP00000116009 antibody|OTTHUMP00000229510 antibody|OTTHUMP00000229511 antibody|OTTHUMP00000229512 antibody|OTTHUMP00000229514 antibody|OTTHUMP00000229515 antibody|OTTHUMP00000229516 antibody|Preprocathepsin B antibody

Gene ID: 1508

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades