Claudin 2 antibody (AA 38-230)

Catalog No. ABIN3042751

Product Details anti-Claudin 2 Antibody

Target: Claudin 2 (CLDN2)

Binding Specificity: AA 38-230

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Claudin-2(CLDN2) detection. Tested with WB, IHC-P in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Claudin-2(CLDN2) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: claudin 2
Protein Name: Claudin-2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for Claudin 2 is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Claudin 2 antibody (ABIN3042751)

Cen, Liu, Li, He, Wang, Liu, Liu, Ji: "Alteration in P-glycoprotein at the blood-brain barrier in the early period of MCAO in rats." in: The Journal of pharmacy and pharmacology, Vol. 65, Issue 5, pp. 665-72, (2013) (PubMed).

Target Details for Claudin 2

Target: Claudin 2 (CLDN2)

Alternative Name: CLDN2 (CLDN2 Products)

Synonyms: CLDN2 antibody, zgc:92260 antibody, AL022813 antibody, RGD1560247 antibody, claudin 2 antibody, CLDN2 antibody, cldn2 antibody, Cldn2 antibody

Background: Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.

Synonyms: Claudin-2 antibody|Claudin2 antibody|CLD2_HUMAN antibody|CLDN 2 antibody|CLDN2 antibody|OTTHUMP00000023793 antibody|SP 82 antibody|SP82 antibody

Gene ID: 9075

UniProt: P57739

Pathways: Hepatitis C