Cofilin antibody (AA 2-166)

Catalog No. ABIN3042747

This anti-Cofilin antibody is a Rabbit Polyclonal antibody detecting Cofilin in WB, IHC, ELISA, IF, FACS, ICC and IHC (fro). Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for Cofilin antibody (AA 2-166) (ABIN3042747)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cofilin Antibody

Binding Specificity: AA 2-166

Purpose: Anti-Cofilin/CFL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cofilin/CFL1 Antibody Picoband® (ABIN3042747). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000). "Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents". Cell Motil. Cytoskeleton 45 (4): 293-306. 3. Lappalainen P, Drubin DG (July 1997). "Cofilin promotes rapid actin filament turnover in vivo".Nature 388 (6637): 78-82.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1

Background:

Synonyms: Cofilin-1,18 kDa phosphoprotein,p18,Cofilin, non-muscle isoform,CFL1,CFL,

Tissue Specificity: Widely distributed in various tissues.

Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH , phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

Sequence Similarities: Belongs to the actin-binding proteins ADF family.

Molecular Weight: 19 kDa

Gene ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events