Cofilin antibody (C-Term)

Catalog No. ABIN3042746

Product Details anti-Cofilin Antibody

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Binding Specificity: AA 122-152, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Cofilin-1(CFL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: DAIKKKLTGI KHELQANCYE EVKDRCTLAE K

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Cofilin-1(CFL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cofilin 1 (non-muscle)
Protein Name: Cofilin-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin (122-152aa DAIKKKLTGIKHELQANCYEEVKDRCTLAEK), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1 (CFL1 Products)

Synonyms: cfl1 antibody, xac1 antibody, xac2 antibody, cfl antibody, cb86 antibody, sb:cb86 antibody, zgc:56501 antibody, wu:fb18a04 antibody, wu:fk77b03 antibody, 12/8 antibody, ADF antibody, ADF/cofilin antibody, Adf antibody, CG4254 antibody, Cadf antibody, D-cof antibody, D61 antibody, Dmel\\CG4254 antibody, T8 antibody, Tsr antibody, cofilin antibody, cofilin/ADF antibody, cofilin/actin depolymerizing factor homolog antibody, l(2)03101 antibody, l(2)k03101 antibody, l(2)k05633 antibody, l(2)k13409 antibody, ntf antibody, CFL antibody, AA959946 antibody, Cof antibody, CFL1 antibody, cofilin 1 (non-muscle) L homeolog antibody, cofilin 1 (non-muscle) antibody, cofilin 1 (non-muscle), like antibody, cofilin 1 antibody, twinstar antibody, cofilin antibody, cofilin 1, non-muscle antibody, Contrafreeloading antibody, cfl1.L antibody, cfl1 antibody, cfl1l antibody, CFL1 antibody, tsr antibody, COF1 antibody, TSTA_042790 antibody, Cfl1 antibody, CFL antibody

Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH , phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

Synonyms: 18 kDa phosphoprotein antibody|CFL 1 antibody|CFL antibody|CFL1 antibody|COF1_HUMAN antibody|Cofilin 1 antibody|Cofilin 1 non muscle antibody|Cofilin antibody|Cofilin non muscle isoform antibody|Cofilin-1 antibody|epididymis secretory protein Li 15 antibody|HEL-S-15 antibody|non-muscle isoform antibody|p18 antibody

Gene ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events