IL-4 antibody (Middle Region)

Catalog No. ABIN3042695

The Rabbit Polyclonal anti-IL-4 antibody has been validated for WB. It is suitable to detect IL-4 in samples from Human. There are 5+ publications available.

Quick Overview for IL-4 antibody (Middle Region) (ABIN3042695)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL-4 Antibody

Binding Specificity: AA 72-98, Middle Region

Purpose: Anti-Interleukin-4 IL4 Antibody Picoband®

Sequence: AATVLRQFYS HHEKDTR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Interleukin-4 IL4 Antibody (ABIN3042695). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human IL-4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Prokopchuk, O., Liu, Y., Henne-Bruns, D., Kornmann, M. Interleukin-4 enhances proliferation of human pancreatic cancer cells: evidence for autocrine and paracrine actions. Br J Cancer.2005 Mar 14,92(5):921-8. 2. Arai, N., Nomura, D., Villaret, D., DeWaal Malefijt, R., Seiki, M., Yoshida, M., Minoshima, S., Fukuyama, R., Maekawa, M., Kudoh, J., Shimizu, N., Yokota, K., Abe, E., Yokota, T., Takebe, Y., Arai, K. Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression. J. Immun. 142: 274-282, 1989.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IL-4 antibody (ABIN3042695)

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Yan, Wang, Liang, Fu, Guo: "HPV16L1-attenuated Shigella recombinant vaccine induced strong vaginal and systemic immune responses in guinea pig model." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 12, pp. 3491-8, (2015) (PubMed).

Yuan, Zhang, Yang: "Ligusticum wallichii Extract Inhibited the Expression of IL-1? after AMI in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 620359, (2014) (PubMed).

Wang, Feng, Shi, Hao, Yang, Zhang, Kong: "Silencing of miR155 promotes the production of inflammatory mediators in Guillain-Barré syndrome in vitro." in: Inflammation, Vol. 36, Issue 2, pp. 337-45, (2013) (PubMed).

Tan, Liu, Yang, Wang, Qin: "An inactivated Pseudomonas aeruginosa medicament inhibits airway allergic inflammation and improves epithelial functions." in: The journal of physiological sciences : JPS, Vol. 63, Issue 1, pp. 63-9, (2012) (PubMed).

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Background:

Synonyms: Interleukin-4,IL-4,B-cell stimulatory factor 1,BSF-1,Binetrakin,Lymphocyte stimulatory factor 1,Pitrakinra,IL4,

Background: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Sequence Similarities: Belongs to the IL-4/IL-13 family.

Molecular Weight: 15 kDa

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation