KIT Ligand antibody (N-Term)

Catalog No. ABIN3042665

Product Details anti-KIT Ligand Antibody

Target: KIT Ligand (KITLG)

Binding Specificity: AA 26-44, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Kit ligand(KITLG) detection. Tested with WB, IHC-P in Human.

Sequence: DEGICRNRVT NNVKDVTKLV

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Kit ligand(KITLG) detection. Tested with WB, IHC-P in Human.
Gene Name: KIT ligand
Protein Name: Kit ligand

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SCF(26-44aa DEGICRNRVTNNVKDVTKLV).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-KIT Ligand antibody (ABIN3042665)

Li, Wu, Miao, Xiong et al.: "Effects of Polysaccharide of Gastrodia Elata Blume and Electro-Acupuncture on Expressions of Brain-Derived Neurotrophic Factor and Stem Cell Factor Protein in Caudate Putamen of Focal Cerebral ..." in: Medical science monitor basic research, Vol. 22, pp. 175-180, (2017) (PubMed).

Jeng, Jeng, Jeng, Sheen, Li, Lu, Chang: "Tropism of liver epithelial cells toward hepatocellular carcinoma in vitro and in vivo with altering gene expression of cancer stem cells." in: American journal of surgery, Vol. 215, Issue 4, pp. 735-743, (2017) (PubMed).

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG (KITLG Products)

Synonyms: Xkl-1 antibody, Xsl antibody, Xsl-1 antibody, Xsl-2 antibody, steel antibody, KITLG antibody, SCF antibody, kitl antibody, kl-1 antibody, mgf antibody, scf antibody, FPH2 antibody, KL-1 antibody, Kitl antibody, MGF antibody, SF antibody, SHEP7 antibody, Clo antibody, Con antibody, Gb antibody, Kitlg antibody, Mgf antibody, SLF antibody, Sl antibody, blz antibody, contrasted antibody, 710-712 antibody, CSF antibody, KITL antibody, KIT ligand L homeolog antibody, KIT ligand antibody, kit ligand antibody, kitlg.L antibody, KITLG antibody, kitlg antibody, Kitl antibody, Kitlg antibody

Background: Stem Cell Factor(also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor(CD117). SCF can exist both as a transmembrane protein and a soluble protein. SCF is localized to the long arm of human chromosome 12, between 12q14.3 and 12qter. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF has been shown to increase the survival of HSCs in vitro and contributes to the self renewal and maintenance of HSCs in-vivo. HSCs at all stages of development express the same levels of the receptor for SCF(c-Kit). The stromal cells that surround HSCs are a component of the stem cell niche, and they release a number of ligands, including SCF.

Synonyms: C kit ligand antibody|C-kit ligand antibody|Ckit ligand antibody|DKFZp686F2250 antibody|familial progressive hyperpigmentation 2 antibody|FPH2 antibody|KIT ligand antibody|Kitl antibody|KITLG antibody|KL 1 antibody|KL1 antibody|Mast cell growth factor antibody|MGF antibody|SCF antibody|SF antibody|SHEP7 antibody|sKITLG antibody|Soluble KIT ligand antibody|Steel factor antibody|Stem cell factor antibody|Stem cell factor precursor antibody

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway